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Showing: 761 - 780 of 8972 results
  1. pCSN061

    Plasmid
    #101725
    Purpose
    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Inserts
    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pJH104

    Plasmid
    #48262
    Purpose
    Template plasmid for PCR-based transplant of Saccharomyces cerevisiae TEF1 promoter in yeast.
    Depositor
    Inserts
    TEF1p 5' fragment
    URA3 5' fragment
    TEF1p 3' fragment
    Use
    Routine cloning vector
    Tags
    Expression
    Mutation
    From SK1 background. -343 to -1 relative to TEF1 …
    Promoter
    TEF1p
    Available since
    Feb. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. pJH124

    Plasmid
    #48259
    Purpose
    Template plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.
    Depositor
    Inserts
    URA3 3' fragment
    URA3 5' fragment
    Ag TEF1 promoter
    Use
    Routine cloning vector
    Tags
    Expression
    Mutation
    -242 upstream to URA3 ORF +495 and URA3 ORF from …
    Promoter
    Ag TEF1 promoter
    Available since
    Feb. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
  4. GTU-c

    Plasmid
    #81093
    Purpose
    Gene Tagging vector URA/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
    Depositor
    Insert
    yemCFP
    Use
    Cre/Lox
    Tags
    yemCFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. pGAPZa-STE2-1D4

    Plasmid
    #133422
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.
    Depositor
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    C59S, C252A
    Promoter
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. Padh1-OsTIR1(WT)-NLS-TADH1-Sp.arg3+/pUC19*

    Plasmid
    #169352
    Purpose
    Expression of OsTIR1(WT) in S. pombe from adh1 promoter, integrated at arg3-D4 locus
    Depositor
    Insert
    OsTIR1(WT)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pAW8-aid-3HA-Turg1:kanMX6

    Plasmid
    #169355
    Purpose
    C-terminal aid-3HA degron cassette with kanMX6 marker for S. pombe
    Depositor
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    3XHA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pETcon(I-LtrI)

    Plasmid
    #41523
    Depositor
    Insert
    I-LtrI
    Use
    Tags
    3x(G4S) Linker, G3S Linker, HA Tag, and Myc Tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
  9. pAW8-aid-V5-Turg1:kanMX6

    Plasmid
    #169357
    Purpose
    C-terminal aid-V5 degron cassette with kanMX6 marker for S. pombe
    Depositor
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    1XV5
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pAW8-aid-V5-eDSR-Turg1:kanMX6

    Plasmid
    #169365
    Purpose
    C-terminal aid-V5-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    1XV5
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pAW8-aid-5Myc-Turg1:kanMX6

    Plasmid
    #169356
    Purpose
    C-terminal aid-5Myc degron cassette with kanMX6 marker for S. pombe
    Depositor
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    5XMyc
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pAW8-aid-3HA-eDSR-Turg1:kanMX6

    Plasmid
    #169363
    Purpose
    C-terminal aid-3HA-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    3XHA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. pAW8-aid-5Myc-eDSR-Turg1:kanMX6

    Plasmid
    #169364
    Purpose
    C-terminal aid-5Myc-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    5XMyc
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. pAW8-aid-3HA-spo5DSR-Turg1:kanMX6

    Plasmid
    #169359
    Purpose
    C-terminal aid-3HA-spo5DSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    3XHA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 761 - 780 of 8972 results