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Showing: 761 - 780 of 15679 results

  1. pTETRIS-cargo

    Plasmid
    #126033
    Purpose
    Luciferase reporter construct for the study of cis-repressive activity in long noncoding RNAs. Also confers puromycin resistance.
    Depositor
    Mauro Calabrese
    Article
    Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do
    Type
    Empty backbone
    Use
    Luciferase; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    126033-plasmid-map-sequence-id-245791

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  2. lentiCRISPR v2-dCas9

    Plasmid
    #112233
    Purpose
    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2
    Depositor
    Thomas Gilmore
    Article
    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do
    Inserts
    dCas9
    Porumycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Changed: Aspartic acid 10 to Glycine, Histidine 8…
    Promoter
    EFS-NS
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112233-plasmid-map-sequence-id-213540

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  3. pLentiGuide

    Plasmid
    #117986
    Purpose
    pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.
    Depositor
    Paul Khavari
    Article
    Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31
    Insert
    sgRNA backbone (F+E)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    117986-plasmid-map-sequence-id-235371

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  5. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

    Plasmid
    #123363
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.
    Depositor
    Sidi Chen
    Article
    Chow et al Nat Methods. 2019 May;16(5):405-408. doi
    Insert
    EGFP-NLS
    Use
    CRISPR, Lentiviral, and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123363-plasmid-map-sequence-id-240469

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  6. pLCKO2

    Plasmid
    #125518
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.
    Depositor
    Jason Moffat
    Article
    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    125518-plasmid-map-sequence-id-248252

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  7. pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE

    Plasmid
    #123362
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.
    Depositor
    Sidi Chen
    Article
    Chow et al Nat Methods. 2019 May;16(5):405-408. doi
    Insert
    Firefly luciferase
    Use
    CRISPR, Lentiviral, and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123362-plasmid-map-sequence-id-240467

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  8. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171991-plasmid-map-sequence-id-338127

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  12. ACE2

    Plasmid
    #164219
    Purpose
    Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker
    Depositor
    Sriram Neelamegham
    Article
    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1
    Insert
    ACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    silent mutation to remove internal EcoRI site (GA…
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    164219-plasmid-map-sequence-id-323632

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  15. PL-SIN-EOS-C(3+)-EiP

    Plasmid
    #21313
    Purpose
    Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance
    Depositor
    James Ellis
    Article
    Hotta et al Nat Methods. 2009 May . 6(5):370-6.
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EOS-C(3+) promoter region
    Available since
    June 1, 2009
    Availability
    Academic Institutions and Nonprofits only
    21313-plasmid-map-sequence-id-9854

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  16. pICE-RNaseHI-WT-NLS-mCherry

    Plasmid
    #60365
    Purpose
    Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Patrick Calsou, Sébastien Britton
    Article
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Insert
    RNase HI
    Use
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60365-plasmid-map-sequence-id-256737

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  17. pEXPqcxip-hFTSJ3-FLAG

    Plasmid
    #165463
    Purpose
    Human FTSJ3 retroviral mammalian expression vector. FLAG/Gateway modified pQCXIP vector with C-terminal FLAG epitope and bicistronic puromycin expression.
    Depositor
    Martin Dorf
    Article
    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11
    Insert
    FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3 Human)
    Use
    Retroviral; Gateway
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/MSV, 5'LTR
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    165463-plasmid-map-sequence-id-327804

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  19. pICE-HA-Ku70-siR-WT

    Plasmid
    #82329
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Patrick Calsou, Sébastien Britton
    Article
    Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10
    Insert
    Ku70 (XRCC6 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-tet
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    82329-plasmid-map-sequence-id-155729

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  20. PL-SIN-EOS-S(4+)-EiP

    Plasmid
    #21314
    Purpose
    Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and Puro resistance
    Depositor
    James Ellis
    Article
    Hotta et al Nat Methods. 2009 May . 6(5):370-6.
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EOS-S(4+) promoter region
    Available since
    June 1, 2009
    Availability
    Academic Institutions and Nonprofits only
    21314-plasmid-map-sequence-id-305770

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Showing: 761 - 780 of 15679 results