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Plasmid#185041PurposeJ23108 promoter, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationOnly first 90 nt of rpmE coding presentPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR089-L36p
Plasmid#185042PurposeJ23108(spel), pHP14 stability hairpin, strong RBS, L36p coding region, trrnBDepositorInsertL36p
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR090-L36
Plasmid#185043PurposeJ23108(spel), pHP14 stability hairpin, strong RBS, L36 coding region, trrnBDepositorInsertL36
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR091-L31p
Plasmid#185044PurposeJ23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region, trrnBDepositorInsertL31p
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pssAAV_Seq17_CMV_bglobin_3NLS_TFP_WPRE_polyA
Plasmid#220223PurposeAAV-STARR-seq validation control vector with TFPDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cut190*_pET21b
Plasmid#215404PurposeExpression construct for Cut190* gene, codon optimized for expression in E. coliDepositorInsertCut190*
UseTagsExpressionBacterialMutationS226P/R228SPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgRNA scaffold-spacer-mouse U6
Plasmid#214698Purposeprovide "sgRNA scaffold-spacer-mouse U6" fragmentDepositorInsertsgRNA scaffold-spacer-mouse U6
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
LacI pm
Plasmid#218097PurposePhagemid that expresses LacI but no phage proteins, used as a controlDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
RoL10X_pET29b
Plasmid#215427PurposeExpression construct for RoL10X gene, codon optimized for expression in E. coliDepositorInsertRoL10X
UseTagsExpressionBacterialMutationV209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…PromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylB-SCY_pET29b
Plasmid#215415PurposeExpression construct for NylB-SCY gene, codon optimized for expression in E. coliDepositorInsertNylB-SCY
UseTagsExpressionBacterialMutationR187S/F264C/D370YPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu
Plasmid#216421PurposeEmpty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTags3xmycExpressionBacterialMutationPromoterAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPbU6_hdhfr/yfcu
Plasmid#216422PurposeEmpty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT (PGK) 12xHRE-NanoLuc
Plasmid#208575Purposehypoxic response element driven NanoLuc Lentiviral reporterDepositorInsertsNanoLuciferase
d2eGFP
12xHRE
UseLentiviral, Luciferase, and Synthetic BiologyTags3x SV40 NLS and PESTExpressionMammalianMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-IRES-puro6 gal4DBD-HIFCAD myc tag
Plasmid#207171Purposeexpression plasmid for Gal4DBD-HIF-CAD.myc expressionDepositorInsertGal4DBD-HIF1a-CAD Myc tag linker
UseTags2xMyc tagExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-IRES-puro6 +gal4DBDHIFCAD pGalO linker
Plasmid#207172Purposeexpression plasmid for Gal4DBD-HIF-CAD expression GalO linkerDepositorInsertGal4DBD-HIF1a-CAD GalO linker
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lneg-INTR-GFP
Plasmid#208902Purposeencodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius neglectus, tagged with GFPDepositorInsertINTR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP
Plasmid#179717PurposeLentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFPDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Drosophila Wdr20-V5
Plasmid#217622PurposeMammalian expression of V5 tagged Drosophila Wdr20DepositorInsertWDR20
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD101
Plasmid#212925PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
GL4.10 Prg4 promoter-luciferase
Plasmid#195034PurposePrg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vectorDepositorInsertPrg4 promoter (PRG4 Bovine)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only