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Showing: 7761 - 7780 of 11898 results

  3. pETM6-G6-vioABE

    Plasmid
    #73438
    Purpose
    Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PG6 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73438-plasmid-map-sequence-id-135337

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  7. Epac-S-H81

    Plasmid
    #170339
    Purpose
    GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.
    Insert
    GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170339-plasmid-map-sequence-id-344640

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  9. pKM50

    Plasmid
    #123656
    Purpose
    Encodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cells
    Depositor
    Kevin Maringer
    Article
    Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0
    Insert
    Aedes aegypti UbL40 promoter
    Use
    Luciferase
    Tags
    Expression
    Insect
    Mutation
    Promoter
    UbL40 promoter
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    123656-plasmid-map-sequence-id-243712

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  10. Polymerase expression - pCMV-ePhi29 (LM2802)

    Plasmid
    #208967
    Purpose
    Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208967-plasmid-map-sequence-id-415814

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  11. pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG

    Plasmid
    #69842
    Purpose
    This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag
    Depositor
    Michel Bornens
    Article
    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.
    Insert
    PLK4 (PLK4 Human)
    Use
    Tags
    3xFLAG tag and EGFP
    Expression
    Mammalian
    Mutation
    K41M-S285A-T289A
    Promoter
    CMV
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    69842-plasmid-map-sequence-id-123881

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  17. CaMKIV-DCTK75E

    Plasmid
    #126423
    Purpose
    A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)
    Depositor
    Douglas Black
    Article
    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi
    Insert
    calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed lysine 75 to glutamic acid
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126423-plasmid-map-sequence-id-249663

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Showing: 7761 - 7780 of 11898 results