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pCW57.1-ZNF746

Plasmid

#207825

Purpose

Dox-inducible expression of ZNF746

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746 (ZNF746 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR2b-mScarlet

Plasmid

#207961

Purpose

Gateway entry vector with the mScarlet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mScarlet

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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p35S::SP-mCherry-GFP-HDEL

Plasmid

#159097

Purpose

Positive control for FRET in plant ER / nuclear envelope.

Depositor

Joe McKenna

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

SP-mCherry-GFP-HDEL

Use

Tags

mCherry, GFP

Expression

Plant

Mutation

Promoter

35S

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRset-rogRFP2

Plasmid

#207875

Purpose

Bacterial expression of the red fluorescent rogRFP2 redox sensor

Depositor

Mathew Tantama

Article

Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

Insert

rogRFP2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GW1-rogRFP2

Plasmid

#207876

Purpose

Mammalian expression of the red fluorescent rogRFP2 redox sensor

Depositor

Mathew Tantama

Article

Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

Insert

rogRFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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MLKS2delARMec

Plasmid

#130860

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Expression

Mutation

Deletion of armadillo domain

Promoter

Available since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKS2del4exp

Plasmid

#130861

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of terminal four amino acids

Promoter

Available since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKS2delKASHexp

Plasmid

#130862

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of KASH domain

Promoter

Available since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKS2delTMexp

Plasmid

#130863

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of transmembrane domain

Promoter

Available since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKS2delARMexp

Plasmid

#130864

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of armadillo domain

Promoter

Available since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKP2delKASH

Plasmid

#130838

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Use

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of KASH domain, addition of three alani…

Promoter

CaMV 35S

Available since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKS2del4ec

Plasmid

#130857

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Expression

Mutation

Deletion of terminal four amino acids

Promoter

Available since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKS2delKASHec

Plasmid

#130858

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Expression

Mutation

eGFP-FLAG-HA

Promoter

Available since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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mAMCase_CatD_6xHis_pTwistCMV

Plasmid

#200228

Purpose

Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pTwist CMV vector

Depositor

James S Fraser

Article

Díaz et al eLife 2023 (Reviewed Preprint)

Insert

Acidic Mammalian Chitinase (Chia1 Mouse)

Use

Tags

6xHis

Expression

Mammalian

Mutation

S354F

Promoter

CMV

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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mAMCase_CatD_6xHis_pcDNA3.1

Plasmid

#200229

Purpose

Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pcDNA 3.1 vector

Depositor

James S Fraser

Article

Díaz et al eLife 2023 (Reviewed Preprint)

Insert

Acidic Mammalian Chitinase (Chia1 Mouse)

Use

Tags

6xHis

Expression

Mammalian

Mutation

S354F

Promoter

CMV

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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RS02060_pMQ30_KO_Kan

Plasmid

#185391

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS02060 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS02060 upstream flanking homology region
RR42_RS02060 downstream flanking homology region

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS28935_pMQ30_KO_Kan

Plasmid

#185392

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS28935 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS28935 upstream flanking homology region
RR42_RS28935 downstream flanking homology region

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS18965_pMQ30_KO_Kan

Plasmid

#185394

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS18965 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS18965 upstream flanking homology region
RR42_RS18965 downstream flanking homology region

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS18970_pMQ30_KO_Kan

Plasmid

#185395

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS18970 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS18970 upstream flanking homology region
RR42_RS18970 downstream flanking homology region

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pJS506

Plasmid

#200712

Purpose

Promoter region from ATPase PMA1 as Pro5U promoter module for heterologous expression of plasma membrane localized transporters in yeast

Depositor

Jens Boch

Article

Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

Insert

PMA1 promoter (PMA1 Budding Yeast)

Use

Synthetic Biology; Modular cloning

Tags

Expression

Mutation

domesticated for MoClo cloning (removal of intern…

Promoter

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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