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Plasmid#89081Purposemammalian expression of human androgen receptor N-terminal fragment 1-660 amino acids linked to VP16 activation domainDepositorInserthAR-1-660 (AR Human)
UseTagsVP16ExpressionMammalianMutationaa 1-660 (contains exons A,B,C and part of D)PromoterSV40Available sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ntng2.b-AP-His
Plasmid#71995PurposeExpresses the extracellular region of the Netrin G2, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNtng2.b (Ntng2 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
BmR1_04g07470-bio-His
Plasmid#108037PurposeExpresses enzymatically monobiotinylated full-length BmR1_04g07470 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertBmR1_04g07470
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
BMR1_02g00320-bio-His
Plasmid#108028PurposeExpresses enzymatically monobiotinylated full-length BMR1_02g00320 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertBMR1_02g00320
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sdk1-Fc-His
Plasmid#72134PurposeExpresses the extracellular region of the Sdk1 protein (endogenous signal peptide replaced with CD33 signal peptide), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSdk1 (Sdk1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
P12-bio
Plasmid#47725PurposeExpresses enzymatically monobiotinylated full-length P12 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised P12
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK cyclin T1 (1-551) (P#422)
Plasmid#14609DepositorInsertcyclin T1 (1-551) (CCNT1 Human)
UseTagsGSTExpressionBacterialMutationamino acids 1-551PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
Sema4d-AP-His
Plasmid#72031PurposeExpresses the extracellular region of the Sema4D protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema4d (Sema4d Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti PA-mCit-GW
Plasmid#113456PurposeLentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusionsDepositorTypeEmpty backboneUseLentiviralTagsProteinA-mCitrineExpressionMammalianMutationPromoterhUbCAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
CLAG3.2-bio
Plasmid#47797PurposeExpresses enzymatically monobiotinylated full-length CLAG3.2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised CLAG3.2
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.5-Fc-His
Plasmid#72102PurposeExpresses the extracellular region of the Neuropilin 2, isoform 5 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNrp2.5 (Nrp2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A24-VN
Plasmid#135508PurposeMammalian expression of VN-fused and myc-tagged HLA-A*24:02DepositorInsertHLA-A*24:02 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
PVX_081550-bio
Plasmid#68532PurposeExpresses full-length P. vivax PVX_081550 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequenceDepositorInsertPVX_081550
UseTagsrat CD4 d3+4, enzymatic biotinylation sequenceExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-MEK5
Plasmid#53167PurposeExpresses HA tagged human MEK5 from blasticidin resistance retroviral vectorDepositorInsertMEK5 (MAP2K5 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
p274 pCMV-CRE-M-AR(T)
Plasmid#12497DepositorInsertCRE-M AR (AR Human)
UseCre/LoxTagsExpressionMammalianMutationEarly termination mutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
ASP-bio
Plasmid#47745PurposeExpresses enzymatically monobiotinylated full-length ASP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised ASP
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
RF-118-UB-BRCA1
Plasmid#78960PurposeExpression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cellsDepositorInsertBRCA1 (BRCA1 Human)
UseTags3x Flag and ubiquitinExpressionMammalianMutationdelta 1-1155PromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
RAP1-bio
Plasmid#47794PurposeExpresses enzymatically monobiotinylated full-length RAP1 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RAP1
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
RhopH2-bio
Plasmid#47798PurposeExpresses enzymatically monobiotinylated full-length RhopH2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RhopH2
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
ABE8.13-d
Plasmid#136297Purposeexpresses ABE8.13-d in mammalian cellsDepositorInsertABE8.13-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only