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pRS313 - prp8 T1872E

Plasmid

#90970

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

T1872E

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 T1861P

Plasmid

#90971

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

T1861P

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 V1862Y

Plasmid

#90972

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

V1862Y

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 H1863E

Plasmid

#90973

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

H1863E

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 K1864E

Plasmid

#90974

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

K1864E

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 N1869D

Plasmid

#90975

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

N1869D

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 V1870N

Plasmid

#90976

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

V1870N

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 I1875T

Plasmid

#90977

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

I1875T

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS1

Plasmid

#145362

Purpose

Cell-free/mammalian expression vector for Zika virus NS1 protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS1

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Availability

Academic Institutions and Nonprofits only

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pmCell-Free_KA2_ZIKV NS2B

Plasmid

#145364

Purpose

Cell-free/mammalian expression vector for Zika virus NS2B protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2B

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS3

Plasmid

#145365

Purpose

Cell-free/mammalian expression vector for Zika virus NS3 protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS3

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Availability

Academic Institutions and Nonprofits only

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pET-17b_K490clm_E215C

Plasmid

#169995

Purpose

Kinesin KIF5B K490 cysteine light mutant with E215C mutation for single cysteine labeling

Depositor

Ron Vale

Article

Niekamp et al Proc Natl Acad Sci U S A. 2019 Mar 5;116

Insert

kinesin family member 5B, isoform CRA_a

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C7S, C65A, C168A, C174S, E215C, C294A, C330S, C42…

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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CN2109-rAAV-eHGT_410m-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#208157

Purpose

Direct-expressing SYFP2 in oligodendrocytes AAV Virus

Depositor

Boaz Levi, The Allen Institute for Brain Science

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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CN3098-pAAV-eHGT_380h-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#208123

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

Boaz Levi, The Allen Institute for Brain Science

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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CN2558-rAAV-3xCore2(390m)-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#208141

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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CN2556-rAAV-3xCore(410m)-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#208144

Purpose

Direct-expressing SYFP2 in oligodendrocytes AAV Virus

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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PB-TAC-ERP2-(N-HA)KRAS_G12D

Plasmid

#201254

Purpose

Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

Depositor

Alexander Kleger

Article

Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

Insert

KRAS (KRAS Human)

Use

Piggybac transposon

Tags

HA-Tag

Expression

Mammalian

Mutation

changed Gly (G) 12 to Asp (D)

Promoter

TRE

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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CN2102-rAAV-eHGT_387m-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#208133

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

Boaz Levi, The Allen Institute for Brain Science

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CN2845-rAAV-eHGT_641m-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#208158

Purpose

Direct-expressing SYFP2 in oligodendrocytes AAV Virus

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CN2157-rAAV-eHGT_395h-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#208146

Purpose

Direct-expressing SYFP2 in oligodendrocytes AAV Virus

Depositor

Boaz Levi, The Allen Institute for Brain Science

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge