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Addgene
Showing: 7721 - 7740 of 16034 results

  1. pcDNA3.1 Flag YFP hNKCC1 C723S C724V W732C (NT546)

    Plasmid
    #50908
    Purpose
    Expresses human NKCC1 C723S C724V W732C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C723S C724V W732C in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    50908-plasmid-map-sequence-id-76381

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  2. HTATIP (2OU2)

    Plasmid
    #33338
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HTATIP (KAT5 Human)
    Use
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. pTP943

    Plasmid
    #104157
    Purpose
    Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse IgG1 Fab specific nanobody TP886
    Use
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    104157-plasmid-map-sequence-id-200077

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  4. pTP1172

    Plasmid
    #104161
    Purpose
    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
    Use
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    104161-plasmid-map-sequence-id-200072

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  5. pTP1174

    Plasmid
    #104162
    Purpose
    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
    Use
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    104162-plasmid-map-sequence-id-200079

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  9. p667-UBC-CDK12-V5-miniTurbo_IDG-K

    Plasmid
    #135243
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5-miniTurbo (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135243-plasmid-map-sequence-id-269114

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  10. p667-UBC-CDK12-V5-TurboID_IDG-K

    Plasmid
    #135244
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5-TurboID (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135244-plasmid-map-sequence-id-311575

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  11. p667-UBC-CDK12-V5_IDG-K

    Plasmid
    #135245
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5 (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135245-plasmid-map-sequence-id-268084

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  12. p667-UBC-BRSK2-V5-miniTurbo_IDG-K

    Plasmid
    #135252
    Purpose
    Gateway destination clone of BRSK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    BRSK2-V5-miniTurbo (BRSK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135252-plasmid-map-sequence-id-269118

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  13. p667-UBC-BRSK2-V5-TurboID_IDG-K

    Plasmid
    #135253
    Purpose
    Gateway destination clone of BRSK2 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    BRSK2-V5-TurboID (BRSK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135253-plasmid-map-sequence-id-269091

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  14. p667-UBC-BRSK2-V5_IDG-K

    Plasmid
    #135254
    Purpose
    Gateway destination clone of BRSK2 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    BRSK2-V5 (BRSK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135254-plasmid-map-sequence-id-268566

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  15. p667-UBC-AAK1-V5-miniTurbo_IDG-K

    Plasmid
    #135255
    Purpose
    Gateway destination clone of AAK1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    AAK1-V5-miniTurbo (AAK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135255-plasmid-map-sequence-id-267913

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  16. p667-UBC-AAK1-V5-TurboID_IDG-K

    Plasmid
    #135256
    Purpose
    Gateway destination clone of AAK1 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    AAK1-V5-TurboID (AAK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135256-plasmid-map-sequence-id-267910

    Enlarge

  17. p663-UBC-V5-CDK13_IDG-K

    Plasmid
    #135268
    Purpose
    Gateway destination clone of CDK13 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-CDK13 (CDK13 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135268-plasmid-map-sequence-id-269140

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  18. p663-UBC-miniTurbo-V5-BRSK2_IDG-K

    Plasmid
    #135271
    Purpose
    Gateway destination clone of BRSK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-BRSK2 (BRSK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135271-plasmid-map-sequence-id-269110

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  19. p663-UBC-TurboID-V5-BRSK2_IDG-K

    Plasmid
    #135272
    Purpose
    Gateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-BRSK2 (BRSK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135272-plasmid-map-sequence-id-269112

    Enlarge

  20. p663-UBC-V5-BRSK2_IDG-K

    Plasmid
    #135273
    Purpose
    Gateway destination clone of BRSK2 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-BRSK2 (BRSK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135273-plasmid-map-sequence-id-269115

    Enlarge

Showing: 7721 - 7740 of 16034 results