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pGH156_MS2-AID-Hygro

Plasmid

#85415

Purpose

lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID and Hygromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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LRT2B-AdGO

Plasmid

#136910

Purpose

Lentivirus expressing AdGO sgRNA

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

sgAdGO

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

hU6

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-TMPRSS11D-G418

Plasmid

#158477

Purpose

Constitutive lentiviral expression of human TMPRSS11D.

Depositor

Alejandro Chavez, Sho Iketani

Insert

TMPRSS11D (TMPRSS11D Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.blast-Flag-MFF-S155,172D

Plasmid

#74442

Purpose

Lentiviral expression vector containing flag tagged S155,172D MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Ser 155 Asp, Ser 172 Asp (Numbering based on MFF …

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only
pLJM1-FH-AGO2-D358K

Plasmid

#91983

Purpose

Expresses FLAG-HA-AGO2 (D358K)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D358K

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PRKDC gRNA (BRDN0001149438)

Plasmid

#77863

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKDC

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKDC (PRKDC Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

Plasmid

#71218

Purpose

dCas9 fused to the human DNMT3A catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3A(E752A)

Use

Lentiviral

Tags

SPdCas9-DNMT3A(E752A)

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-TALE1

Plasmid

#27969

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-puro_TAOK1

Plasmid

#209777

Purpose

doxycycline-inducible expression of TAOK1

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

TAOK1 (TAOK1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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7TF

Plasmid

#24306

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

7xTcf-FFluc

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB2-S310Y

Plasmid

#116350

Purpose

Lentiviral expression of ERBB2 S310Y

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Tags

Expression

Mutation

S310Y

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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FUG+p97W (C2)

Plasmid

#74018

Purpose

lentiviral expression of EGFP-SAP97beta fusion

Depositor

Weifeng Xu, Oliver Schluter, Robert Malenka

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

SAP97 beta isoform

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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EF1a_LMX1A_P2A_Hygro_Barcode

Plasmid

#120457

Purpose

Barcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

LMX1A (LMX1A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-SIK1

Plasmid

#138695

Purpose

Expresses a human SIK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK1 human (SIK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgSIK2

Plasmid

#138708

Purpose

Expresses a human SIK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 human (SIK2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-mIFP-NLS

Plasmid

#173893

Purpose

for mIFP expression in nucleus

Depositor

Ron Vale

Article

Yan et al J Cell Biol. 2021 Feb 1;220(2). pii: 211

Insert

mIFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SSFV

Available since

Nov. 23, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2653

Plasmid

#144059

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ARNTL2 (BMAL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0685

Plasmid

#143428

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PRDM10 (PRDM10 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2255

Plasmid

#141994

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ARNTL (BMAL1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag

Plasmid

#199457

Purpose

Expression of dCas13-VPR in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

dCas13

Use

Lentiviral

Tags

VPR-T2A-HaloTag

Expression

Mammalian

Mutation

Nuclease-dead H133A and H1058A

Promoter

CAG

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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