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pYC50

Plasmid

#63906

Purpose

Recipient integrative vector to construct MYC tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Use

Tags

MYC

Expression

Yeast

Mutation

Promoter

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pMS22H

Plasmid

#133904

Purpose

Expression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET17

Plasmid

#107443

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET17 (MET17 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pKH-MoHyPer

Plasmid

#78267

Purpose

Express HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.

Depositor

Jeffrey Caplan, Nicole Donofrio

Article

Huang et al Mol Plant Pathol. 2016 Mar 7. doi: 10.11

Insert

MoHyper

Use

Express in magnaporthe

Tags

Expression

Yeast

Mutation

Promoter

Rp27

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only
pWS2247

Plasmid

#123026

Purpose

Tet promoter (TetO(x7)-pLEU2m)

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pTet [2]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTCW012

Plasmid

#83527

Purpose

Cloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminator

Depositor

Claudia Vickers

Article

Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

pFUS1J2

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pDS250

Plasmid

#34969

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Use

Tags

Expression

Yeast

Mutation

V416I

Promoter

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET5

Plasmid

#107437

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET5 (MET5 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

Promoter

GAL1

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRS303 Bt δCOP

Plasmid

#73871

Purpose

Bos taurus Arcn1

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2 Promoter- COPD

Use

Tags

Expression

Yeast

Mutation

Promoter

Ret2 Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pRG714_lexO-AscI_LexA-TF_URA3MX

Plasmid

#154822

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Use

Tags

FLAG and NLS

Expression

Yeast

Mutation

Promoter

ACT1 and lexO_4-CYC1_core

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6-mTFP1-His3MX6

Plasmid

#69850

Purpose

C-terminal tagging with mTFP1 using His3MX6 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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GGE020

Plasmid

#120777

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position1-3. When using the GG platform for expressing only one gene (T1-3_Lip2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T1-3_Lip2

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1-YFP

Plasmid

#18685

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pRC063

Plasmid

#123057

Purpose

Blue fluorescent protein

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

mTagBFP2 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCP5

Plasmid

#35397

Depositor

Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Type

Empty backbone

Use

Reporter

Tags

Expression

Yeast

Mutation

Promoter

GDP promoter

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pCMVTnT-Flp-3xFLAG

Plasmid

#109167

Purpose

Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a mammalian expression system

Depositor

Monika Golas

Article

Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

Insert

Flp

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pFTK086

Plasmid

#171358

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

P-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-vps10-104

Plasmid

#115426

Purpose

Generate the vps10-104 allele via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

vps10-104

Use

Tags

Expression

Yeast

Mutation

domain 1 deletion

Promoter

Endogenous

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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POM42-mCitrine

Plasmid

#69854

Purpose

N-terminal tagging with mCitrine using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCitrine minus start and stop codon

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1

Plasmid

#18684

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only