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Plasmid#188502PurposeExpresses FLAG tagged AsdCas12a KRABDepositorInsertdCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationD908APromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP3-Halo
Plasmid#175310PurposeProduction of HaloTag fusion proteinDepositorInsertIGF2BP3 (IGF2BP3 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mt-ro1GFP
Plasmid#82407PurposeExpression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cellsDepositorInsertro1GFP
UseTagsleader sequence of the E1α subunit of pyruvate de…ExpressionMammalianMutationC48S/F64L/T65S/S147C/Q204CPromoterCMVAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMH1105
Plasmid#131864PurposeProduces holo-PhyB-AviTag in E. coliDepositorInsertsPhyB(1-651)-AviTag-His6
HO1
PcyA
UseTagsExpressionBacterialMutationPromoterT7 promoter-1 and T7 promoter-2Available sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUB_smFLAG_ActB_MS2
Plasmid#81083PurposeTranslation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_ActB_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_Puro
Plasmid#167871PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
G1BgSVNA-CDK4
Plasmid#16573DepositorInsertCDK4 (CDK4 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAUC40
Plasmid#71298PurposeSuicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXCas9H840A
Plasmid#60900PurposeDual Expression Vector for Cas9 H840A nickase and gRNADepositorTypeEmpty backboneUseCRISPRTags3XFLAGExpressionMammalianMutationPromoterCBh and U6Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRT-KanR-FRT pA
Plasmid#82601Purpose3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selectionDepositorInsertFRT-Kan-FRT
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJOE6090.1
Plasmid#135080PurposepBR322-based L-rhamnose-inducible vector.DepositorInsertP-rhaP-BAD-Strep-tag II-eGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-FLAG-hEcto/Tif1g
Plasmid#20902DepositorInsertEctodermin (TRIM33 Human)
UseTagsFLAGExpressionMammalianMutationN-terminal deletion of 37 aminoacids; Ectodermin …PromoterAvailable sinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
GFP-C2gamma
Plasmid#21215DepositorInsertGFP-pcDNA3-PKCgamma-c2 (Prkcg Rat)
UseTagsGFPExpressionMammalianMutationThe C2-domain from PKCgamma (aa170-260) plus flan…PromoterAvailable sinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_Blasto
Plasmid#167864PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_Puro
Plasmid#167863PurposePiggyBac compatible plasmid expressing spCas9DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4137384
Plasmid#149543PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike) for VSV pseudotypingDepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationH. sapiens recodePromoterCMVAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
FRB-polySH33-EGFP
Plasmid#175792PurposeBacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantificationDepositorInsertNCK1 (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromotertacAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-AcHELIX-KanR (CJT96)
Plasmid#181794PurposepDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mito-Lapronic/pGW1
Plasmid#140754PurposeFRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate RatioDepositorInsertMito-Lapronic
UseTagsMitochondrial Sequence SignalExpressionMammalianMutationPromoterCMVAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
p255 pPr-CREM/Ins/CMV-STOP-luc
Plasmid#8408DepositorInsertCREM/insulators/lox/luciferase
UseCre/LoxTagsExpressionMammalianMutationmodified CrePromoterAvailable sinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only