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Plasmid#149561PurposeflaB gRNA expression in B. burgdorferiDepositorInsertsgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNArodA
Plasmid#149569PurposerodA gRNA expression in B. burgdorferiDepositorInsertsgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
R2x2_VAL88
Plasmid#160761PurposeExpresses R2x2_VAL88 in bacterial cellsDepositorInsertR2x2_VAL88
UseTags6xHisExpressionBacterialMutation10-residue mutations of Rsmn2x2_5_6PromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_P0826S-sgRNA500
Plasmid#149618PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterP0826SAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PresTL-sgRNA500
Plasmid#149617PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPresTLAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXW101Plux2-Pecf11-gfp
Plasmid#160823PurposeTwo-layered AHL-responsive circuit carrying an ECF11-based amplifierDepositorInsertPJ23101-30luxR-t-Plux2-33ecf11-Pecf11-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPJ23101, Plux2, Pecf11Available sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNA500
Plasmid#149559PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-11a (D3 - L39V)
Plasmid#136607PurposeExpresses DANCER-3 mutant (reversion L39V)DepositorInsertDANCER-3 mutant (L39V reversion)
UseTags5x-HistagExpressionBacterialMutationMutation from GB1 WT: Y3F-L7I-A34F-V54IPromoterT7 promoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-11a (D3 - I54V)
Plasmid#136608PurposeExpresses DANCER-3 mutant (reversion I54V)DepositorInsertDANCER-3 mutant (I54V reversion)
UseTags6x-HistagExpressionBacterialMutationMutation from GB1 WT: Y3F-L7I-A34F-V39LPromoterT7 promoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-11a (D3 - F34A)
Plasmid#136606PurposeExpresses DANCER-3 mutant (reversion F34A)DepositorInsertDANCER-3 mutant (F34A reversion)
UseTags6x-HistagExpressionBacterialMutationMutation from GB1 WT: Y3F-L7I-V39L-V54IPromoterT7 promoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only