Addgene

We narrowed to 9,011 results for: yeast

Showing: 7681 - 7700 of 9011 results

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pYC46

Plasmid

#63904

Purpose

Recipient integrative vector to construct FLAG tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pRS414 GPD-Sis1

Plasmid

#18687

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 (SIS1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pRS267

Plasmid

#122380

Purpose

CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Insert

HSP90

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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KBB285

Plasmid

#185076

Purpose

RFA3-GFP fusion

Depositor

Ken Belanger

Article

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

RFA3

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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KBB284

Plasmid

#185075

Purpose

RFA2-GFP fusion

Depositor

Ken Belanger

Article

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

RFA2

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMJ273: pRS426-His-MBP-GtFz1_PSP1

Plasmid

#205258

Purpose

S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ127: pRS426-His-MBP-NlovFz2_PSP1

Plasmid

#205257

Purpose

S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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POM42-mTFP1

Plasmid

#69852

Purpose

N-terminal tagging with mTFP1 using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1 minus start and stop codon

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRG645_lexO-AscI_LexA-TF_LEU2MX

Plasmid

#154813

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Use

Tags

FLAG and NLS

Expression

Yeast

Mutation

Promoter

ACT1 and lexO_4-CYC1_core

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pL-rpb1-N488D

Plasmid

#10988

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-N488D (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 1462 A to G in the RPB1 ORF resulting in…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pWS1037

Plasmid

#123049

Purpose

G_ subunit

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Gpa1 [3-4a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pYC119

Plasmid

#63916

Purpose

Recipient overexpressing vector to construct FLAG tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

TEF1 (Saccharomyces cerevisiae)

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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BiFC1

Plasmid

#190046

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172)

Use

Tags

Expression

Yeast

Mutation

Promoter

Met3

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pYC48

Plasmid

#63905

Purpose

Recipient integrative vector to construct HA tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pYC54

Plasmid

#63909

Purpose

Recipient integrative vector to construct YFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pFTK093

Plasmid

#171365

Purpose

Vector part (LVL1) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

sgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSSN6

Plasmid

#104340

Purpose

Express SSN6

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

Insert

SSN6 (CYC8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pWS627

Plasmid

#123064

Purpose

G___ bicistronic protein

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste4-2A-Ste18 [3-4a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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POM43-mCherry

Plasmid

#69857

Purpose

N-terminal tagging with mCherry using LEU2 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCherry minus start and stop codon

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pYC52

Plasmid

#63908

Purpose

Recipient integrative vector to construct CFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Use

Tags

CFP

Expression

Yeast

Mutation

Promoter

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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