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Plasmid#142458PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZEB2 (ZEB2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-HA
Plasmid#162100PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to VSVg-StrepTagII-HA
UseLentiviralTagsVSVg-StrepTagII-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G
Plasmid#157984PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G
Plasmid#157985PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
FCK-Chronos-GFP
Plasmid#80902PurposeLenti-mediated expression of Chronos-GFP under the CaMKII promoter.DepositorInsertChronos-GFP
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCaMKIIAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2alpha WT
Plasmid#128812PurposeTo make virus expressing Doc2alpha WTDepositorInsertDoc2alpha (Doc2a Rat)
UseLentiviralTagsIRES-EGFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta WT
Plasmid#128814PurposeTo make virus expressing Doc2beta WTDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsIRES-EGFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-H13LTat-CD8a/d2eGFP-IRES-Nef
Plasmid#126552PurposeReplication incompetent HIV with H13L Tat and d2eGFP/CD8a reporterDepositorInsertHIV-1 vector pNL4-3
UseLentiviralTagsCD8a and GFPExpressionMutationDelta gag/polPromoterHIV LTRAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
eGFP Depdc5 pLJM1
Plasmid#46380DepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagseGFPExpressionMammalianMutationDiffers from Depdc5 isoform 1 by 10 amino acids …PromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJZC48
Plasmid#62336PurposesgRNA + 1x COM with COM-VP64 effector for mammalian cellsDepositorInsertssgRNA + 1x COM binding module
COM-VP64
UseLentiviralTagsVP64ExpressionMammalianMutationTargets Tet3G, sequence: GTACGTTCTCTATCACTGATAPromoterCMV and U6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CCNB1-P2A-Hygro_Barcode
Plasmid#170218PurposeBarcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCCNB1 (CCNB1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
CHUK gRNA (BRDN0001149372)
Plasmid#77033Purpose3rd generation lentiviral gRNA plasmid targeting human CHUKDepositorInsertCHUK (CHUK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CHUK gRNA (BRDN0001149269)
Plasmid#77034Purpose3rd generation lentiviral gRNA plasmid targeting human CHUKDepositorInsertCHUK (CHUK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4
Plasmid#193348PurposetetO-S*K (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4)DepositorUseLentiviralTagsExpressionMammalianMutationSox2-17 is engineered highly cooperative chimeric…PromoterTetO mini CMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLCAG EGFP
Plasmid#14857DepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-NLS-tdMCP-mCherry
Plasmid#99909PurposeFor the expression of NLS-tdMCP-mCherryDepositorInsertNLS-tdMCP-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
ERN2 gRNA (BRDN0001145316)
Plasmid#75845Purpose3rd generation lentiviral gRNA plasmid targeting human ERN2DepositorInsertERN2 (ERN2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro-Chen-tracrRNA_2xBsmBI
Plasmid#196710PurposeBackbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choiceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0665
Plasmid#141656PurposeLentiviral vector for overexpressing the SCX transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSCX (SCXB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
SHP2(T253M/Q257L)@pLKO-Trex-SBP
Plasmid#85458PurposeSHP2 mutant gene expressionDepositorInsertSHP2 (PTPN11 Human)
UseLentiviralTagsSBP TagExpressionMammalianMutationT253M/Q257LPromoterRSVAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only