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pCF142_U6-sgRNA

Plasmid

#225960

Purpose

U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

Depositor

Christof Fellmann

Article

Ramadoss et al Nat Commun. 2025 Nov 17;16(1):9883. doi:

Insert

U6-sgRNA (recipient)

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

Plasmid

#114068

Purpose

T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for LbCas12a crRNAs

Use

In vitro transcription

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-FURIN

Plasmid

#158386

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

furin, paired basic amino acid cleaving enzyme (FURIN Human)

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRSFDuet-v2 RsTAL At4CL

Plasmid

#126524

Purpose

expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

Depositor

Andrew Woolley

Article

Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

Inserts

tyrosine ammonia lyase
p-coumaroyl-CoA ligase (4CL1 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV14-3X-Flag-SARS-CoV-2 S

Plasmid

#145780

Purpose

Over expression of SARS-CoV-2 S (spike) in mammalian cell line

Depositor

Zhaohui Qian

Article

Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi:

Insert

SARS-CoV-2 S (S SARS CoV 2)

Tags

3xFlag

Expression

Mammalian

Mutation

Codon optimized and C-terminal 19 amino acids del…

Promoter

CMV

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHIV-IL13-GAPGlySer-PDGFR

Plasmid

#240247

Purpose

Lentiviral transfer plasmid encoding IL13 with GAP-Gly-Ser linker and PDGFR TM

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-eDHFR-Cdc42Q61L

Plasmid

#107267

Purpose

mCherry-eDHFR-Cdc42Q61L in pIVT vector for in vitro transcription. Note that Cdc42 in this plasmid keeps its CAAX domain.

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

mCherry-eDHFR-Cdc42Q61L (CDC42, dhfr Human)

Tags

mCherry

Expression

Mammalian

Promoter

T7

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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ACE2

Plasmid

#164219

Purpose

Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker

Depositor

Sriram Neelamegham

Article

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Mutation

silent mutation to remove internal EcoRI site (GA…

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

Plasmid

#104140

Purpose

Expresses TREAT-5'TOP reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Tags

24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

Expression

Mammalian

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ230-RR

Plasmid

#108860

Purpose

LbCpf1 Gateway entry plasmid with G548R and K611R mutations

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

LbCpf1-RR (LbCpf1 with G548R and K611R mutations)

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

G548R and K611R, Cpf1 is rice codon optimized

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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