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pCM271rfp

Plasmid

#59774

Purpose

pCMrfp with TrfA mutation (271R to C)

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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HHM-iATPSnFR1.0

Plasmid

#102546

Purpose

expresses ATP sensor in bacteria

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pNC-BiFC-ED4

Plasmid

#193429

Purpose

Nimble cloning-compatible vector for plant BiFC

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->myc-mtC11L34

Plasmid

#162226

Purpose

Lentiviral vector for the expression of Myc mtC11L34 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-PNE scFv C11L34

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Yellow

Plasmid

#160443

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Yellow

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pN3-Sp3FL

Plasmid

#24541

Depositor

Guntram Suske

Article

Sapetschnig et al J Biol Chem. 2004 Oct 1. 279(40):42095-1

Insert

transcription factor Sp3 (SP3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-T7-TALEN(Sangamo)-Destination

Plasmid

#37184

Purpose

Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

Depositor

Pawel Pelczar

Article

Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

Type

Empty backbone

Use

Tags

FokI nuclease

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->GssGFP-P2A-NLSIFP

Plasmid

#162235

Purpose

Lentiviral vector for the expression of TRE3GS-->GssGFP-P2A-NLSIFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

GFP - IFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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PSICORhp-mp53

Plasmid

#26273

Depositor

Rudolf Jaenisch

Article

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

p53 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
pJPC12-ΔM13-PRM-B0034-geneVI

Plasmid

#80858

Purpose

Accessory plasmid (encodes M13 gene VI)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Insert

M13 gene VI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lambda Prm

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) VSFP3.1

Plasmid

#18951

Depositor

Thomas Knopfel

Article

Lundby et al PLoS ONE. 2008 . 3(6):e2514.

Insert

VSFP3.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2008

Availability

Academic Institutions and Nonprofits only

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Opto-hEGFR_308

Plasmid

#58746

Purpose

Expresses Opto-hEGFR (light-activated receptor tyrosine kinase)

Depositor

Harald Janovjak

Article

Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

Insert

Opto-hEGFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ134-tRNA

Plasmid

#179213

Purpose

Golden Gate entry vector; 4th gRNA with tRNA

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

no promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBC2103-reci

Plasmid

#117172

Purpose

U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_arCas9 without NLS

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pP2-phiV10

Plasmid

#196333

Purpose

Plasmid expressing chimeric P2-phiV10 tail fiber

Depositor

Baojun Wang

Article

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

chimeric tail fiber P2-phiV10

Use

Tags

Expression

Bacterial

Mutation

Promoter

P2 late promoter

Available since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-Kan-alphaGal4

Plasmid

#13421

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

RNAP alpha-Gal4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pKTrfp

Plasmid

#59772

Purpose

pKT230 derivative; PBAD_rfp; cmR

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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LP538

Plasmid

#90202

Purpose

pCMV-myc with cDNA encoding HsTRAPPC8 full length

Depositor

Lotte Pedersen

Article

Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

Insert

TRAPPC8 (TRAPPC8 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

full length protein, no mutations

Promoter

CMV

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-p63RhoGEF

Plasmid

#67896

Purpose

Expresses a fusion of mCherry with full-length p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

mCherry-p63RhoGEF

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBT224_(pCA-tTA2)

Plasmid

#36430

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tetracycline transactivator

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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