Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 7641 - 7660 of 145654 results

  5. pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53205
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and kanMX6
    Expression
    Mutation
    Promoter
    Gal1 promoter
    Availability
    Academic Institutions and Nonprofits only

  6. pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53206
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and TRP1
    Expression
    Mutation
    Promoter
    GAL1 promoter
    Availability
    Academic Institutions and Nonprofits only

  7. pFA6a-HIS3MX6-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53207
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and HIS3MX6
    Expression
    Mutation
    Promoter
    GAL1 promoter
    Availability
    Academic Institutions and Nonprofits only

  12. {Ud}RpL14.Dm

    Plasmid
    #53220
    Purpose
    {Ud} is composed of two genes; (1) a UAS-RpL14.dsRNA targeting RNAi to a haploinsufficient gene RpL14 and (2) an RNAi insensitive RpL14 rescue
    Depositor
    Diethard Tautz
    Insert
    RpL14 (RpL14 Fly)
    Use
    Attb drosophila melanogaster transformation
    Tags
    Expression
    Mutation
    14 synonymous mutations introduced
    Promoter
    endogenous promoter
    Availability
    Academic Institutions and Nonprofits only
    53220-plasmid-map-sequence-id-82863

    Enlarge

  15. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid
    #53225
    Purpose
    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
    Depositor
    Jesse Rinehart
    Insert
    MEK1 (MAP2K1 Human)
    Use
    Phosphoprotein synthesis
    Tags
    HIS and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli, both codons for Ser21…
    Promoter
    pLtetO
    Availability
    Academic Institutions and Nonprofits only
    53225-plasmid-map-sequence-id-82901

    Enlarge

  17. pSK-mPar1

    Plasmid
    #53227
    Purpose
    bacterial expression of mouse Par1
    Depositor
    Shaun Coughlin
    Insert
    Par1 (F2r Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  19. SK-hPar3

    Plasmid
    #53229
    Purpose
    bacterial expression of human Par3
    Depositor
    Shaun Coughlin
    Insert
    Par3 (F2RL2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  20. pBJ-mPar3

    Plasmid
    #53230
    Purpose
    mammalian expression of mouse Par3
    Depositor
    Shaun Coughlin
    Insert
    Par3 (F2rl2 Mouse)
    Use
    Tags
    FLAG and bovine prolactin signal sequence (MDSKGS…
    Expression
    Mammalian
    Mutation
    Deletion of aa1-15 (native signal peptide)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 7641 - 7660 of 145654 results