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pGWB402

Plasmid

#74796

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-IGK-dAPEX2-KDEL

Plasmid

#117183

Purpose

Plasmid name in publication: pAAV-DIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

IGK-dAPEX2-KDEL

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pML104-KanMx4

Plasmid

#83476

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

Depositor

Patricia Wittkopp

Insert

KanMX4

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM28

Plasmid

#124960

Purpose

Mammalian expression of Human TRIM28

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pINDUCER11 (miR-RUG)

Plasmid

#44363

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV_AncBE4max_P2A_GFP

Plasmid

#112100

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

AncBE4max_P2A_GFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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hIRE1a wt

Plasmid

#20744

Depositor

Fumihiko Urano

Article

Lipson et al PLoS ONE. 2008 . 3(2):e1648.

Insert

IRE1 (ERN1 Human)

Use

Lentiviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf1(WT)-HA

Plasmid

#79409

Purpose

Expresses C-terminally HA-tagged Arf1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf1 (ARF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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CRY2(535, L348F)-mCherry

Plasmid

#75372

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 residues 1-535 (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

L348F

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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mc2 mNeon hPGK

Plasmid

#206230

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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