-
Plasmid#199465PurposeExpression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.DepositorInsertGal4-ALFA-tag-VP16
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET14-GFP30_Encap
Plasmid#86406PurposeExpresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 30aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRAIP-SFB
Plasmid#101769PurposeMammalian expression of TRAIP fusion proteinDepositorInsertTRAIP / RNF206 (TRAIP Human)
UseTagsS peptide-flag-streptavidin binding peptide (SFB)ExpressionMammalianMutationsiRNA-resistant allelePromoterAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-3xFLAG-NLS-GFP
Plasmid#198238PurposeLentivector with tet-inducible 3xFLAG-NLS-GFPDepositorInsert3xFLAG-NLS-GFP
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterTREAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHBV-Luc
Plasmid#71414PurposeTo see the activity of HBV transcription using Luciferase assayDepositorInsertHBV core promoter, enhancer I, enhancer II and luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterHBVAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEntr1a-wGBD-mTurquoise2-wGBD
Plasmid#176136PurposeVisualization of active Cdc42DepositorInsertWAS (WAS Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQTEV- PRDX2
Plasmid#31338DepositorInsertPRDX2 (PRDX2 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
OPN(-1206)-luc
Plasmid#31106DepositorInsertosteopontin promoter (SPP1 Human)
UseLuciferaseTagsExpressionMammalianMutationpromoter region -1206 to +87PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGR
Plasmid#46002PurposeReporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinoseDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHBV-X/EnhI-Luc
Plasmid#71418PurposeTo see HBV X promoter(overlaped EnhanceI) activity for luciferase assayDepositorInsertHBV X promoter and enhancer I driving Luciferase expression
UseLuciferaseTagsExpressionMammalianMutationPromoterHBV XAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1X-Nup88
Plasmid#87338PurposeTo express human Nup88 in mammalian cellsDepositorInsertNUP88 (NUP88 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDA077
Plasmid#131388PurposeL1RP expressionDepositorInsertL1RP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
RIP Cre-ER (DM#265)
Plasmid#15029DepositorInsertRat insulin promoter (RIP) (Ins2 Rat)
UseCre/Lox; For making transgenic constructsTagsCre-ER (TM)ExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-Cre-6xHis
Plasmid#62939PurposeExpression of Cre-6xHis in bacterial of cellsDepositorInsertCre recombinase
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pORE303N
Plasmid#194438PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, KpnI site for gRNA insertionDepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationPromoter2x35SAvailable sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDAS12395_PEAR-GFP_2.0-preedited
Plasmid#177185PurposePEAR-GFP3 control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
BcLOV-mCh-EGFR
Plasmid#208274PurposeExpresses BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.DepositorInsertBcLOV-mCherry-EGFR
UseLentiviralTagsmCherryExpressionMutationPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A
Plasmid#102585PurposeControl lentivector. Coexpression of mTagBFP and gene of interest from SFFV promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-OT (wt TCF-4 sites)
Plasmid#16558DepositorInsertTCF-4 binding sites (TCF4 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutation3 copies of wildtype Tcf-4 binding sitesPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046852
Plasmid#145584PurposeBacterial Expression plasmid for SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits