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Plasmid#133813PurposeCRISPR-Cas9 system for genetic manipulation of Candida tropicalisDepositorInsertcassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMW#3
Plasmid#13350DepositorInsertLacZ reporter
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCS4333 yeTadA1.0-T7 RNAP
Plasmid#137735PurposepCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable markerDepositorInsertyeTadA1.0-T7 RNAP
UseTagsExpressionBacterial and YeastMutationE366G (See depositor comment below)PromoterAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVG122
Plasmid#211765PurposeExpresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.DepositorInsertstrongLOV
UseFor single-copy integration (if cut with pmei)TagsSV40 NLS and VP16ExpressionYeastMutationcompared to EL222, strongLOV contains Glu84Asp mu…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRG705_lexO-AsiSI_LexA-TF_LEU2MX
Plasmid#154817PurposeInducible expression of AsiSI endonuclease in S. cerevisiaeDepositorInsertsAsiSI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAP-BAC
Plasmid#120229PurposeTAR cloning in yeast and low-copy maintenance of large constructs in E. coliDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-Mitotrap
Plasmid#46942PurposeExpresses Mitotrap in mammalian cellsDepositorInsertMitotrap (MTOR Human, Synthetic, Aequorea victoria, Budding Yeast)
UseTagsThis is a fusion between a yeast mitochondrial ta…ExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKL1-222
Plasmid#219409PurposeNuclear expression of Synrophin PDZ clamp proteinDepositorInsertpTEF1-NLS-(Syntrophin-PDZ)x4-tADH1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec611
Plasmid#130872PurposeA nuclear plasmid encoding an error-prone mutant TP-DNAP1 (I777K, L900S) for OrthoRepDepositorInsertTP-DNAP1
UseTagsExpressionYeastMutationI777K, L900SPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS423-TyrSgH
Plasmid#163973PurposeHelper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pADH99
Plasmid#90979PurposeNAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.DepositorInsertsUS_HIS1
FRT
pENO1
C. albicans Cas9
pMAL2
FLP
NAT 1 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYC34
Plasmid#63915PurposeRecipient overexpressing vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneUseTagsmCherryExpressionYeastMutationPromoterTEF1 (Saccharomyces cerevisiae)Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG646_lexO-HO_LexA-TF_LEU2MX
Plasmid#154815PurposeInducible expression of HO endonuclease in S. cerevisiaeDepositorInsertsUseTagsFLAGExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
eGFP L202 gRNA
Plasmid#119132PurposegRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.DepositorInserteGFP L202 gRNA
UseCRISPRTagsExpressionMutationNonePromoterU6Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-HALO-3xHA
Plasmid#188937PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-HALO-3xHA
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Vrg4-V5-APEX2-URA
Plasmid#207071PurposeExpressing Vrg4-V5-APEX2. Marker for late Golgi/early endosomes.DepositorInsertVrg4
UseTagsV5-APEX2ExpressionYeastMutationPromoterpVRG4Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pADH140
Plasmid#90988PurposeNAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.DepositorInsertsC. maltosa LEU2 1 of 2
pENO1
Cas9
NAT 1 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH137
Plasmid#90986PurposeNAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.DepositorInsertsC. albicans LEU2 1 of 2
pENO1
Cas9
NAT 1 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYES2-Ub-M-GFP
Plasmid#11952DepositorInsertUb-M-GFP
UseTagsExpressionYeastMutationUbiquitin fused to N-terminus of GFP. Methionine …PromoterAvailable sinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fSNAP-3xHA
Plasmid#188935PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fSNAP-3xHA
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only