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pGMC00014
Plasmid#172526Purposenon targeting sgRNADepositorInsertNon-targeting guide
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-dLight1.2
Plasmid#111069PurposeTo generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoterDepositorHas ServiceAAV1InsertdLight1.2
UseAAVTagsFlag tagExpressionMutationPromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DHX36iso2E335A
Plasmid#159588PurposeExpress N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cellsDepositorInsertDHX36 (DHX36 Human)
UseTagsFLAG HAExpressionMammalianMutationE335APromoterCMVAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGLS3-5xHRE-PHLDA3(wt)
Plasmid#72557PurposeHypoxia induced mutant PHLDA3. Contains the HRE from VEGF sequence (5 repeats) upstream of PHLDA3.DepositorInsertPHLDA3 (PHLDA3 Human)
UseTags5XHREExpressionMammalianMutationPromoterE1b minimalAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR3c-S249C
Plasmid#45713DepositorInsertFGFR3 IIIc (FGFR3 Human)
UseRetroviralTagsExpressionMammalianMutationS249CPromoterAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AviMta3-3xFiBsd
Plasmid#140962Purposefull-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cellsDepositorInsertmetastasis associated 3 (Mta3 Synthetic, Mouse)
UseTags3xFLAG, Avi, and TEVExpressionMammalianMutationPromoterCAGAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-155-5p
Plasmid#103265PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-155-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-155-5p target (MIR155 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDest27-mBIN1+17
Plasmid#61096PurposeExpress N-terminal GST tagged-mouse BIN1+17 in mammalian cellsDepositorInsertmouse BIN1 with exon 17, excluding exon 7, 11, 13-16
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-IS186
Plasmid#140627PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot9_18-285_S
Plasmid#147480PurposeMammalian Expression of HsNot9_18-285DepositorInsertHsNot9_18-285 (CNOT9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot9_18-285_S
Plasmid#147482PurposeMammalian Expression of HsNot9_18-285DepositorInsertHsNot9_18-285 (CNOT9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-HsAIDSc
Plasmid#60810Purposeto express human AID recoded for yeast codon usageDepositorInsertAID (AICDA Human)
UseTagsMycExpressionYeastMutationcodon optimized for yeastPromoterGAL1Available sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1318-1605_W
Plasmid#147893PurposeMammalian Expression of HsNot1iso1_1318-1605DepositorInsertHsNot1iso1_1318-1605 (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBpuro - HA KLF4 FL
Plasmid#34589DepositorInsertKLF4 (KLF4 Human)
UseRetroviralTagsHA tagExpressionMammalianMutationPromoterLTRAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcdna DSG-2 TS
Plasmid#89465PurposeDSG-2 tension sensor, using TSmod inserted in human DSG-2DepositorInsertdesmoglein-2 (DSG2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSI-535
Plasmid#131130PurposegRNA expression vector for C-to-T base editing reporterDepositorInsertC-to-T reporter activation gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-q-pep
Plasmid#47437PurposeFRET sensor for beta2-adrenergic receptorDepositorInsertbeta2-adrenergic receptor (ADRB2 Human)
UseTags10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…ExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129G
Plasmid#36049DepositorInsertalpha-synuclein S129G (SNCA Human)
UseTagsExpressionBacterialMutationS129GPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Kir2.1Mut-2A-mScarlet-KASH
Plasmid#203841Purposenon-functional Kir2.1(mutant) expression as a control for the silencing of neurons with Kir2.1 and mScarlet-KASH expressionDepositorInsertKir2.1 (Kcnj2 Mouse)
UseAAVTags2A-mScarlet-KASHExpressionMutationG144A, Y145A, G146APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only