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Plasmid#67619PurposeA pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technologyDepositorTypeEmpty backboneUseIn vitro transcription or translation off of an s…TagsC-terminal FlagExpressionMammalianMutationPromoterCMV and SP6Available sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only
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pcDNA4 huZAP(S)
Plasmid#45906DepositorInsertZAP(S) isoform (ZC3HAV1 Human)
UseTagsHAExpressionMammalianMutationM202T, L388FPromoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-gp78-FLAG
Plasmid#62370PurposeExpress C-terminally FLAG tagged gp78DepositorInsertgp78 (AMFR Human)
UseTagsFLAGExpressionMammalianMutation*PromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
LZ10 PBREBAC-H2BHalo
Plasmid#91564Purposepiggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.DepositorInserthistone H2B-HaloTag
UseTagsHaloTagExpressionMammalianMutationPromoterEF1Available sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL-10_IRES_mCherry_PGK_tBFP
Plasmid#85427PurposeLentiviral vector for Gal4 inducible humanIL-10 with mCherry reporter and constitutive tBFP expressionDepositorInserthuman IL-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI HA NEDD4L
Plasmid#27000DepositorInsertNEDD4L (NEDD4L Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
Flag-TRIM24
Plasmid#28138DepositorInsertTRIM24 (TRIM24 Human)
UseTagsFLAGX2ExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Olig1
Plasmid#64841PurposeA lentiviral vector for the expression of mouse Olig1 under doxycyline controlDepositorInsertOlig1 (Olig1 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}
Plasmid#201120PurposeRFP expression in cells under EF-1α promoter; Histone-bound, nuclearDepositorInsertTurboRFP; H2B-RFP
UseLentiviralTagsExpressionMutationNoPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pScCas9
Plasmid#117700PurposeMammalian expression vector encoding WT ScCas9DepositorInsertStreptococcus canis Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 - TRC mTagBFP2
Plasmid#191566PurposeExpresses an shRNA and mTagBFP2DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNAAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL3-pegRNA-attL2
Plasmid#213052PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI H2B-GFP
Plasmid#92399PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.DepositorInsertIRES H2B EGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
α-actinin-4-M522-Venus in pcDNA6
Plasmid#190802PurposeImaging of α-actinin-4 in live cellsDepositorInsertalpha-actiinin-4-M522-venus (ACTN4 Synthetic, Human)
UseTagsHis, myc, and venusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
SRC_HUMAN_D0
Plasmid#79700PurposeThis plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSRC (SRC Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5/DEST-VPS35
Plasmid#21691DepositorInsertVacuolar Protein Sorting 35 homolog (VPS35 Human)
UseLentiviral; Invitrogen gateway destination vectorTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2)_zeo insert c-MYC
Plasmid#164636PurposesgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64DepositorInserttarget sequence for c-Myc activation (MYC Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMPRAv3:minP-GFP
Plasmid#109036PurposeMPRA v3 minimal promoter with GFP donorDepositorInsertemGFP
UseTagsExpressionMammalianMutationPromoterminPAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
PresentER-SIINFEKL (mCherry)
Plasmid#102945PurposePresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)DepositorInsertSIINFEKL (OVAL Chicken, Synthetic)
UseRetroviralTagsExpressionMammalianMutationOVA257-264PromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pRK5-HA-C12orf66
Plasmid#87048Purposemammalian expression of C12orf66DepositorInsertC12orf66 (C12orf66 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF1344
Plasmid#143792PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFEZF2 (FEZF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-eSpCAS9-NG-pA
Plasmid#131466PurposeExpresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized eSpCas9-NG
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP3
Plasmid#141257PurposeGateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorUseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MX1_pCSdest
Plasmid#53951DepositorInsertMX1 (MX1 Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pdCas9::BFP-humanized
Plasmid#44247PurposeExpression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdownDepositorInsertdCas9 fused to BFP
UseCRISPR and RetroviralTags2xNLS & tagBFPExpressionMammalianMutationD10A H840A (catalytically inactive)PromoterMSCV LTR promoterAvailable sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
CN1528-rAAV-hsA2-eHGT_082h-minRho-SYFP2-WPRE3-BGHpA
Plasmid#163502PurposeDirect-expressing SYFP2 AAV VirusDepositorInsertSYFP2
UseAAVTagsExpressionMutationPromoterMinRhoAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 1-242
Plasmid#24391DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 1-242.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
V5-BS2-NES (Cytosol)
Plasmid#220341PurposeMammalian expression vector BS2 in cytoplasmDepositorInsertBS2
UseTagsNES (Nuclear exclusion sequence) and V5 tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF1939
Plasmid#141893PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMEIS1 (MEIS1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only