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Plasmid#64991PurposeMammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)DepositorInsertOrp1 (HYR1 Budding Yeast)
UseRetroviralTagsroGFP2ExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCMV6-AN-HA_WT POLK
Plasmid#201584PurposeExpresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cellsDepositorInsertPolymerase kappa (POLK Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-NF1-GRD
Plasmid#19993DepositorInsertNeurofibromatosis type I (NF1 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9-P2A-puro
Plasmid#214358PurposeExpresses SpCas9 and puromycin resistance genesDepositorInsertSpCas9 and puromycin resistance genes
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
BPK2660
Plasmid#70709PurposeHuman expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mNFM
Plasmid#32909PurposeMammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of mouse neurofilament protein MDepositorInsertEGFP-mouse NFM fusion (Nefm Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAIDA1
Plasmid#79180PurposeA construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tagsDepositorInsertAdhesin Involved in Diffuse Adherence
UseTagsHis and MycExpressionBacterialMutationPromoterlacUV5Available sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_membrane_EGFP
Plasmid#139402Purposein vitro transcription for mRNADepositorInsertmembrane-EGFP
UseIn vitro transcriptionTagsEGFPExpressionMutationPromoterT3Available sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX313 MSLN
Plasmid#193617PurposeExpresses MSLNDepositorInsertMSLN (MSLN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP670-N1
Plasmid#136556PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertemiRFP670
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-eGFP
Plasmid#32548DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
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PpyCAG-NANOG-IB
Plasmid#60440PurposeTransient human NANOG expressionDepositorInsertNANOG (NANOG Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEDA5_GFPmut3_Y66H
Plasmid#80085PurposePositive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.DepositorInsertGFPmut3_Y66H
UseTags6x-His tagExpressionBacterialMutationchanged Tyrosine 66 to HistidinePromoterproBAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9n(D10A)-Blast
Plasmid#63593PurposeExpresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertsCas9
Blasticidin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationD10A, nickase mutantPromoterEFS-NSAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-ClpX
Plasmid#89657PurposePlasmid for titrating synthesis of ClpX with doxycyclineDepositorInsertclpX
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK18sB
Plasmid#177838PurposePlasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.DepositorTypeEmpty backboneUseBacterial gene replacementTagsExpressionMutationPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
PBMBP-eGFP
Plasmid#40976DepositorInsertMBP promoter fragment (Mbp Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDae079 (eMutaT7[transition])
Plasmid#187622PurposeExpresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertsTadA-8e-T7RNAP
PmCDA1-T7RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only