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pAS_4xMx PylT C41CA_GFP 150TAG

Plasmid

#140017

Purpose

Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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PB-HD118

Plasmid

#172655

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD118A/pegRNA-HD118B

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT sfGFP 150TAG

Plasmid

#154771

Purpose

plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-MGT4-d2EGFP_CMV-mCherry

Plasmid

#213764

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

MGT#4-d2EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-CLGT3-d2EGFP_CMV-mCherry

Plasmid

#213766

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

CLGT#3-d2EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

Plasmid

#206213

Purpose

PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

Depositor

Masamichi Kamihira

Article

Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

Insert

tetracycline-responsive transactivator

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPV-C1-crRNA-cloning-EF1a-BA

Plasmid

#204623

Purpose

piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1067B

Plasmid

#200252

Purpose

pBac-U6-gRNA(doublesex)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

4 gRNAs targeting Doublesex

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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Antares2-N1

Plasmid

#120869

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

Akaluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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PB-HD252

Plasmid

#172656

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD252A/pegRNA-HD252B

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

Plasmid

#162820

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF9c/14

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF9c/14(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)

Plasmid

#162822

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF3b(CoV1h)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF3b(CoV1h)(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Opie2-PuroR[dsxM]

Plasmid

#131616

Purpose

Expresses a functional PuroR only in males and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Inserts

PuroR[dsxM] (dsx Fly)
dsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Opie2 and hr5Ie1

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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BII-C3H2B

Plasmid

#133387

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-gR-PnGW

Plasmid

#133355

Purpose

Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications), co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Use

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP

Plasmid

#124181

Purpose

piggyBac-based reporter (Puro) driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

IRES-Pac, Pou5f1 enhancer

Use

Reporter

Tags

Expression

Mutation

Promoter

UBCmin

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP

Plasmid

#124180

Purpose

piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

EGFP-IRES-Pac, Pou5f1 enhancer

Use

Reporter

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pB[nos-Cas9 PUb-ZsGreen]

Plasmid

#169011

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Tags

Expression

Insect

Mutation

Promoter

Drosophila melanogaster nanos

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-GFP-HYGRO

Plasmid

#115144

Purpose

Safe harbor site 231 knock-in vector with GFP-HygromycinR expression cassette

Depositor

Michael Phelps, Raymond Monnat

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-RFP-HYGRO

Plasmid

#115145

Purpose

Safe harbor site 231 knock-in vector with RFP-HygromycinR expression cassette

Depositor

Michael Phelps, Raymond Monnat

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

RFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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