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Plasmid#165506PurposeType II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.DepositorInsertEco31I
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-TthpolA
Plasmid#165533PurposeDNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C).DepositorInsertTth DNA polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Bstpol
Plasmid#165543PurposeFull length DNA polymerase from Bacillus stearothermophilus.DepositorInsertBst DNA Polymerase, Full Length
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 9, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-T4 PGD
Plasmid#165550PurposeT4 pyrimidine DNA glycosylase; bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities.DepositorInsertT4 PGD (T4 Endonuclease V)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pWPI-myogenin
Plasmid#79073PurposeLentiviral plasmid that constitutively expresses human myogeninDepositorInserthuman myogenin (MYOG Human)
UseLentiviralTagsExpressionMutationPromoterEF1 alphaAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOpen-9N7polA
Plasmid#165500PurposeThermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activityDepositorInsert9 degrees N-7 DNA polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 26, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-T4-BGT
Plasmid#165530PurposeT4 Phage beta-glucosyltransferasetransfers the glucose moiety of UDP-Glc to the 5-hmC residues in double-stranded DNA.DepositorInsertT4-BGT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-ALPI
Plasmid#165555PurposeCIAP reporter; can be used in spectrophotometric or luminescence assay when appropriate substrate is usedDepositorInsertCIP (calf intestinal phosphatase)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-SP6rpol
Plasmid#165516PurposeDNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.DepositorInsertSP6 RNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-DpnIR
Plasmid#165529PurposeUsed to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)DepositorInsertDpnI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-T7EI
Plasmid#165540PurposeDNA Endonuclease; catalyzes cleavage of DNA mismatches and non-beta DNA structures (Holliday junctions and cruciform).DepositorInsertT7EI (T7 Endonuclease I)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 9, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-TEV protease
Plasmid#165509PurposeUsed for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/GDepositorInsertTEV protease
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-NotIR (GC)
Plasmid#165507PurposeType II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.DepositorInsertNotI (GC-rich recognition site)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-PstIM
Plasmid#165536PurposeMethylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.DepositorInsertM.PstI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-9N7polA (CT)
Plasmid#165502Purpose9 Degrees North DNA polymerase variant with enhanced ability to incorporate modified substratesDepositorInsert9 degrees N-7 DNA Polymerase chain terminating
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-EcoRIM
Plasmid#165515PurposeMethylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.DepositorInsertM.EcoRI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-HSlig4
Plasmid#165503PurposeLigase used in efficiently joining single-strand breaks in a double-strand polydeoxynucleotide in an ATP-dependent reaction.DepositorInsertDNA Ligase IV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-RNAse H
Plasmid#165575PurposeEndonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication.DepositorInsertRNAse H
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-K12polLF
Plasmid#165511PurposeDNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.DepositorInsertDNA Polymerase I, Large (Klenow) Fragment
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-GBDpol
Plasmid#165501PurposeRobust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences.DepositorInsertPyrococcus Sp. Heat-Stable DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 26, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits