Addgene

We narrowed to 14,719 results for: grna

Showing: 741 - 760 of 14719 results

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MDC1 KO sgRNA

Plasmid

#207103

Purpose

pX330 based plasmid for expression of Cas9 and the GGTGTAACGTGGAGCCAGTA sgRNA to target the MDC1 coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GGTGTAACGTGGAGCCAGTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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ATG13 sgRNA

Plasmid

#207558

Purpose

pX330 expressing Cas9 and a sgRNA targeting the ATG13 locus

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

ggaaactgatctcaattccc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNAs-Crym

Plasmid

#200067

Purpose

For the knock down of Crym with an astrocyte specific mCherry expression

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

U6-3xsgRNA-Crym

Use

AAV, CRISPR, and Mouse Targeting

Tags

GfaABC1D-mCherry

Expression

Mutation

Promoter

U6, GfaABC1D

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-GFP

Plasmid

#200068

Purpose

For the knock down of GFP with an astrocyte specific mCherry expression

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

U6-sgRNA-GFP

Use

AAV, CRISPR, and Mouse Targeting

Tags

GfaABC1D-mCherry

Expression

Mutation

Promoter

U6, GfaABC1D

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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ATG9A sgRNA

Plasmid

#207557

Purpose

pX330 expressing Cas9 and a sgRNA targeting the ATG9A locus

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

CACTGAATACCAGCGCCTAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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HaloTag KO sgRNA

Plasmid

#207102

Purpose

pX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTCGATGTTGGTCCGCGCGA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #2

Plasmid

#207106

Purpose

pX330 based plasmid for expression of Cas9 and the CTGAAGGAACAGACTAATTC sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CTGAAGGAACAGACTAATTC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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53BP1 KO sgRNA

Plasmid

#207104

Purpose

pX330 based plasmid for expression of Cas9 and the AGATTCTCAGCCTGAAAGCC sgRNA to target the 53BP1 coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AGATTCTCAGCCTGAAAGCC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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WIPI2 sgRNA

Plasmid

#207554

Purpose

pX330 expressing Cas9 and a sgRNA targeting the WIPI2 locus

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

CGCGCGCCCAGCCATGAACC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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SOX2-3'gRNA

Plasmid

#215543

Purpose

gRNA targeting SOX2 3' terminal for CRISPR-Cas9-mediated knock-in

Depositor

Kai Wang

Insert

SOX2 (SOX2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-hcr3

Plasmid

#193660

Purpose

Expression of tandem pre-sgRNA array hcr3 for LbCas12a

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

U6-CFTR-sgRNA-KLF4-sgRNA-TET1-sgRNA-tRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-hcr5

Plasmid

#193661

Purpose

Expression of tandem pre-sgRNA array hcr5 for LbCas12a

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

U6-DNMT3B-sgRNA-KLF4-sgRNA-TET1-sgRNA-PRR5L-sgRNA-CFTR-sgRNA-tRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-pcr4

Plasmid

#193662

Purpose

Expression of tandem pre-sgRNA array pcr4 for LbCas12a

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

U6-PUM1-sgRNA-GHR-sgRNA-HMGA2-sgRNA-PUM2-sgRNA-tRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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INS-3'gRNA

Plasmid

#210468

Purpose

gRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-in

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

INS (INS Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_2

Plasmid

#210622

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_1

Plasmid

#210621

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_4

Plasmid

#210624

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_3

Plasmid

#210623

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-LUC7L2_sgRNA1

Plasmid

#201602

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L2 (LUC7L2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PGM2_sgRNA2

Plasmid

#201617

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

PGM2 (PGM2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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