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pCMV-HAT1_AG

Plasmid

#217113

Purpose

Mammalian expression vector for expression of HAT1_AG transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

HAT1_AG transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP3fast

Plasmid

#74422

Purpose

Express fluorescent calcium indicator GCaMP3fast in mammalian cells

Depositor

Katalin Torok

Article

Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

Insert

GCaMP3fast

Use

Tags

Expression

Mammalian

Mutation

W4Y D356A

Promoter

CMV

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLV.CMVenh.gp91.eGFP.cHS4

Plasmid

#30471

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 May 5. ():.

Insert

CMVenh/gp91/eGFP/cHS4

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-dCpf1-BE

Plasmid

#107685

Purpose

Expresses dLbCpf1-BE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE

Use

Tags

Expression

Mammalian

Mutation

D832A/E925A/D1148A

Promoter

CMV

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-BE-VQRCas9

Plasmid

#101740

Purpose

expression of an optimized Cas9 for base-editing in zebrafish

Depositor

Shuo Lin

Article

Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

Insert

rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, VQR mutations

Promoter

CMV

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tre

Plasmid

#123134

Purpose

Mammalian expression vector for Tre.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Tre recombinase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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HCMV UL97

Plasmid

#26687

Depositor

Robert Kalejta

Article

Kuny et al PLoS Pathog. 2010 . 6(9):.

Insert

UL97 (UL97 Human Cytomegalovirus (HHV-5))

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-R-Hras

Plasmid

#180574

Purpose

ddRFP sensor of Hras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Hras

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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EF.mHES1.CMV.GFP

Plasmid

#17622

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

HES1 (Hes1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV5 HA-TGFBRII

Plasmid

#19158

Depositor

Joan Massague

Insert

Transforming growth factor, beta receptor II (TGFBR2 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

PCR based mutagenesis

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hDDX50-HA

Plasmid

#165465

Purpose

Human DDX50 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DExD-box helicase 50 (DDX50 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-WWC2

Plasmid

#172995

Purpose

constitutive expression of FLAG-tagged WWC2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC2 (WWC2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-BACH1t_RFP

Plasmid

#199214

Purpose

BACH1 C-terminus truncated isoform - BACH1t expression plasmid with a RFP reporter on the backbone.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Inserts

BACH1t (BACH1 Human)
RFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and PGK

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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HA-E2F-1 wt-pRcCMV

Plasmid

#21667

Depositor

William Kaelin

Article

Krek et al Cell. 1994 Jul 15. 78(1):161-72.

Insert

E2F-1 (E2F1 Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

wild type-

Promoter

Available since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV5-CBFbeta

Plasmid

#12427

Depositor

Scott Hiebert

Article

Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

Insert

CBF beta (Cbfb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only
pCCl-MCS_miniCMV_EGFP

Plasmid

#134984

Purpose

The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

minCMV

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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Halo_aTubulin-pTwistCMVBetaGlobWPRENeo

Plasmid

#221901

Purpose

Expresses Halo7 fused to alpha tubulin

Depositor

Janelia Integrative Imaging

Insert

Halo7-aTubulin

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-msfGFP

Plasmid

#180328

Purpose

mammalian expression of human SEPT9_i1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.4

Plasmid

#111057

Purpose

Expresses dLight1.4 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.4

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Wnk1

Plasmid

#38780

Depositor

Melanie Cobb

Article

Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

Insert

Wnk1 (Wnk1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only