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M-NMn-VP64
Plasmid#48676PurposeMammalian NM-VP64 nuclease-null Cas9 activator expression, human optimizedDepositorInsertCas9-VP64, nuclease-null
UseCRISPRTagsNLS and VP64ExpressionMutationnuclease-nullPromoterCMVAvailable sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
#1.4 post-R-SynapShot-Lyn-miRFP670
Plasmid#214718PurposeExpresses red #1.4 post-SynapShot and Lyn-miRFP670DepositorInsert#1.4 post-R-SynapShot
UseTagsLyn-miRFP670ExpressionMammalianMutationPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_Blasto
Plasmid#167864PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK1009 (pSL9)
Plasmid#191004PurposeExpresses E2 Crimson on a broad host range pBBR1 origin plasmidDepositorInsertE2-Crimson
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterCP25Available sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH-CMV-GFP
Plasmid#133017Purpose(Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under CMV promoter.DepositorTypeEmpty backboneUseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
YSBE 516 : pNEO_4xFLAG
Plasmid#92082PurposePlasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, Neo selectionDepositorInsert4xFlag
Usetagging proteins in cryptococcus neoformanTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH2217
Plasmid#179251PurposePsra-11::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherryDepositorInsertTWK-18 (twk-18 Nematode)
UseTagswCherryExpressionWormMutationM280IPromoterPsra-11Available sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMZ-176
Plasmid#195424PurposeTransient, constitutive expression of synZiFTR: ZF1-p65DepositorInsertpUb - 3XFLAG - NLS - ZF1-3 (ZF1) - p65 - RbGpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p8092 LentiCRISPR v2 sgNT-1
Plasmid#163315PurposeExpresses Cas9 and a non-targeting control guide RNADepositorInsertsgNT-1
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS-393
Plasmid#195473PurposeLentiviral Integratable Inducible super-IL2 cytokine + synZiFTR10DepositorInsertinverted[8X ZF10 BS - ybTATA - IL12] - pSFFV - ZF10 - NS3 - p65
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-miR-9 sponge
Plasmid#25040DepositorInsertmiR-9 sponge
UseRetroviralTagsd2EGFPExpressionMammalianMutationPromoterAvailable sinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Pidd1(T788A)
Plasmid#60529PurposeRetroviral expression of Pidd1 T788A phosphorylation mutantDepositorInsertPidd1 (PIDD1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationThreonine 788 to Alanine*PromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-PGK-EGFP
Plasmid#21316PurposeControl Lentiviral vector with ubiquitous PGK promoter, expresses EGFPDepositorInsertEnhanced Green Fluorescence Protein
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGene-TAP-GBP1
Plasmid#138087PurposePlasmid with TAP-GBP1 ORFDepositorInserthuman GBP1 (GBP1 Human)
UseTagsTandem affinity purification (TAP) tagExpressionMammalianMutationPromoterGal4-E1bAvailable sinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
p6618 MSCV-IP N-HAonly WHSC2
Plasmid#35014DepositorInsertWHSC2 (NELFA Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceFeb. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_mcherry
Plasmid#167862PurposePiggyBac compatible plasmid expressing spCas9DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-FB-E25TAG
Plasmid#176545PurposeExpresses IgG affinity protein FB with a TAG stop codon at E25 positionDepositorInsertIgG affinity protein
UseTagsHis tagExpressionBacterialMutationchange glutamic acid 25 to amber stop codonPromoterT5Available sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
YSBE 517 : pNEO_6xHA
Plasmid#92083PurposePlasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, Neo selectionDepositorInsert6xHA
Usetagging proteins in cryptococcus neoformanTagsExpressionMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-K-I-N-CAAX
Plasmid#41640PurposeExpresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activationDepositorInsertDronpa145K-IntersectinDH-Dronpa145N-CAAX
UseTagsCAAX boxExpressionMammalianMutationK145N on DronpaPromoterCMVAvailable sinceDec. 21, 2012AvailabilityAcademic Institutions and Nonprofits only