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pJT282 Lenti pEF-dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast

Plasmid

#187337

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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Lck-mTurquoise2

Plasmid

#98822

Purpose

In vivo visualization of the plasma membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

Lck

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pHes7-Achilles-Hes7

Plasmid

#153528

Purpose

Expresses fusion protein of Achilles/YFP and mouse Hes7 from mouse Hes7 promoter. The reporter shows oscillatory expression with 2-3 periodicity when expressed in mouse presomitic mesoderm.

Depositor

Ryoichiro Kageyama

Article

Yoshioka-Kobayashi et al Nature. 2020 Apr;580(7801):119-123. doi:

Insert

Hes7 (Hes7 Mouse)

Use

Tags

Achilles

Expression

Mammalian

Mutation

Promoter

mouse Hes7 promoter

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-R2Tg_opt-3xFlag

Plasmid

#223251

Purpose

Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein_opt

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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AB-GFP

Plasmid

#72203

Purpose

screen AB42 variants for aggregation

Depositor

Michael Hecht

Article

Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

Insert

AB42 (APP Human)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-STIM1

Plasmid

#228952

Purpose

Retroviral expression of mCherry-STIM1

Depositor

David Pellman

Article

Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED

Plasmid

#219658

Purpose

Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Empty SEED Cassette (3xP3-dsRed)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFPC1 MOSPD1

Plasmid

#226404

Purpose

Expression of human MOSPD1 fused to EGFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

Insert

MOSPD1 (MOSPD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hDlx_DIO_ChR2_mCherry

Plasmid

#224459

Purpose

An AAV vector expressing ChR2-mCherry that is dependent on both Cre and the hDlx enhancer

Depositor

Gordon Fishell

Article

Wu et al bioRxiv 2024.07.20.604399

Insert

ChR2-mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST-v2-PermE

Plasmid

#209446

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

PtipA; sgRNA expression from ermE* promoter

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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PX458 pSp Cas9 Rhino1 tagA

Plasmid

#211627

Purpose

Cas9 Rhino1 tagA

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

RHNO tagA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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MIGR1_FLAG_TAL1-long_GFP

Plasmid

#210638

Purpose

Overexpression of TAL1-long

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-short (TAL1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ZAK (ZAKA-c057)

Plasmid

#98225

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ZAKA (MAP3K20 Human)

Use

Baculovirus

Tags

His-6-TEV

Expression

Mutation

pfam00069:Pkinase 16-257

Promoter

polyhedrin

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C9orf72

Plasmid

#83437

Purpose

Expresses GFP-C9orf72 in mammalian cells

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

C9orf72 (C9orf72 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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VCP(R155H)-EGFP

Plasmid

#23972

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Use

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

R155H

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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CSNK1A1_pLX307

Plasmid

#98326

Purpose

Lentiviral expression of CSNK1A1

Depositor

Sefi Rosenbluh, William Hahn

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen

Plasmid

#182387

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-His

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3 GCN1-3xFlag_IRES-iRFP

Plasmid

#198388

Purpose

GCN1 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDAC435

Plasmid

#195238

Purpose

Mammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU6-29

Plasmid

#66203

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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