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Plasmid#78283PurposemRFP1 expressed behind an ATc-inducible constructDepositorInsertTet inducible mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR2b-K660E
Plasmid#45705DepositorInsertFGFR2 IIIb (FGFR2 Human)
UseRetroviralTagsExpressionMammalianMutationK660EPromoterAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV_eA3A-nCas9
Plasmid#163535PurposeMammalian CG-to-GC base editingDepositorInserteA3A-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDest27-mBIN1+13+17
Plasmid#61097PurposeExpress N-terminal GST tagged-mouse BIN1+13+17 in mammalian cellsDepositorInsertmouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/GUG-DAP5-3XFLAG
Plasmid#127330PurposeExpress 3xFLAG tagged DAP5 protein from GUG start codonDepositorInsertDAP5 (EIF4G2 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4/hL3MBTL2
Plasmid#28232DepositorInsertl(3)mbt-like 2 (Drosophila) (L3MBTL2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR2b-K660N
Plasmid#45707DepositorInsertFGFR2 IIIb (FGFR2 Human)
UseRetroviralTagsExpressionMammalianMutationK660NPromoterAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3XFLAG-wtGATA6-3XAU1 neo
Plasmid#72618PurposeExpresses an inducible, N-terminally 3X-FLAG tagged and C-terminally 3X-AU1 tagged wtGATA6DepositorInsertGATA binding protein 6 (GATA6 Human)
UseLentiviralTags3XAU1 and 3XFLAGExpressionMammalianMutationPromoterTRE TightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G
Plasmid#157986PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGem-sHyper:sAPX
Plasmid#84738PurposeSimultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cellsDepositorInsertsUseTagsnuclear localisation signals from the coding sequ…ExpressionPlantMutationPromoterpFMV/p35SAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330 Human 3' HP1a gRNA
Plasmid#127907PurposeWT Cas9 Vector targeting the 3' end of the human HP1a geneDepositorInsertgRNA for Human 3' HP1a
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Bmx-SH2
Plasmid#46409DepositorInsertBmx (BMX Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 286-400PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Txk-SH2
Plasmid#46526DepositorInsertTxk (Txk Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 138-258PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTwist-FLAG-SRSF3_Full.Length_Codon.Optimized
Plasmid#172345PurposeExpression of FLAG-tagged, codon-optimized, full-length SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseTagsFLAGExpressionMammalianMutationcodon-optimizedPromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_SOX2
Plasmid#45270DepositorInsertMCOPS3 (SOX2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGem-nHyPer:sAPX
Plasmid#84735PurposeSimultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cellsDepositorInsertsUseTagsnuclear localisation signals from the coding sequ…ExpressionPlantMutationPromoterFMV/35SAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-NpHR3.3-EYFP
Plasmid#137151PurposeViral expression of NpHR3.3-EYFPDepositorHas ServiceAAV8InsertNpHR3.3-EYFP
UseAAVTagsExpressionMutationW179FPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRepair-SGFP2-CTNNB1
Plasmid#153430PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsSGFP2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-331-3p
Plasmid#103444PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-331-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-331-3p target (MIR331 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV_RBMX-eA3A-UdgX-nCas9
Plasmid#163546PurposeMammalian CG-to-GC base editingDepositorInsertRBMX-eA3A-UdgX-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only