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Plasmid#103392PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29a-5p target (MIR29A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_SKIL
Plasmid#45265DepositorInsertSNON (SKIL Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HcRed
Plasmid#25892DepositorInserthcRed
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40
Plasmid#98928PurposeCre dependent AAV expression of Ruby2 spaghetti monster FLAG constructDepositorHas ServiceAAV1InsertRuby2-smFP-Flag
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
J23100-mRFP1-331Bb
Plasmid#78271PurposemRFP1 behind constitutive promoter J23100 in pSEVA331Bb backboneDepositorInsertJ23100-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-wtGATA6-3XAU1
Plasmid#72612PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6DepositorInsertGATA binding protein 6 (GATA6 Human)
UseEntry vector for gateway cloningTags3XAU1 and 3XFLAGExpressionBacterialMutationPromoterTRE tight promoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM-shRnd3
Plasmid#86437Purpose2nd generation doxycycline-inducible lentiviral vector expressing Rnd3-targeting shRNA and GFPDepositorInsertshRNA targeting RND3 (RND3 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HF-BE3
Plasmid#87439PurposeExpresses HF-BE3 with C-terminal NLS in mammalian cellsDepositorInsertHF-BE3 (Apobec1 Bacillus phage PBS2, Human, Rat)
UseTagsExpressionMammalianMutationMammalian codon-optimized Cas9-HFPromoterCMVAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJMP3
Plasmid#79875PurposeBacillus subtilis sgRNA expression vector; integrates into thrCDepositorInsertsgRNA RR1
UseCRISPRTagsExpressionBacterialMutationPromotervegAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4/hL3MBTL1
Plasmid#28231DepositorInsertl(3)mbt-like 1 (Drosophila) (L3MBTL1 Human)
UseTagsFLAGExpressionMammalianMutationIrrelevant S49T mutationPromoterAvailable sinceFeb. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMG81-MBP-t-M.MpeI-N374K-His
Plasmid#197985PurposeExpresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.DepositorInsertMBP-t-M.MpeI-N374K-His
UseTagsHis and MBPExpressionBacterialMutationM.MpeI-N374KPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Myc-E-Syt2
Plasmid#66834PurposeMammalian Expression, Bacterial ExpressionDepositorInsertE-Syt2 (ESYT2 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-Med25
Plasmid#64771PurposeMammalian protein expression of flag-tagged Med25DepositorInsertMed25 (MED25 Human)
UseTagsFlagExpressionMammalianMutationPromoterpCMVAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA,3Q)-NLS
Plasmid#192445PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.DepositorInsertmCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA,3Q)
Plasmid#192444PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.DepositorInsertmCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR3c-WT
Plasmid#45711DepositorInsertFGFR3 IIIc (FGFR3 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_TNIK
Plasmid#45276DepositorInsertTNIK (TNIK Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-SMYD3
Plasmid#85650Purposeretroviral expression of human SMYD3DepositorInsertSMYD3 (SMYD3 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterLTRAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_Promoter_1-3
Plasmid#210629PurposeLuciferase experiment for TAL1 promoter 1-3DepositorInsertpromoter 1-3 (TAL1 Human)
UseTagsExpressionMammalianMutationPromoterMinimal PromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only