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Showing: 7421 - 7440 of 14407 results

  11. pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

    Plasmid
    #198054
    Purpose
    All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning
    Depositor
    Lacra Bintu
    Article
    Ludwig et al Cell Syst. 2023 Jun 21;14(6):482-500.e8.
    Inserts
    rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
    3xFLAG-insert
    Use
    Lentiviral and Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G and pEF
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    198054-plasmid-map-sequence-id-391373

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  13. BAP1 C91A

    Plasmid
    #81025
    Purpose
    Retroviral vector containing human BAP1 C91A (catalytically inactive mutant) and Thy1.1 selection marker
    Depositor
    Anjana Rao
    Article
    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.
    Insert
    BAP1 (BAP1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    BAP1 C91A (point mutation, catalytically inactive)
    Promoter
    5’LTR
    Available since
    Nov. 17, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. pcDNA3_erGAP3

    Plasmid
    #78118
    Purpose
    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum
    Depositor
    Javier García-Sancho, Teresa Alonso
    Article
    Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9
    Insert
    erGAP3
    Use
    Tags
    KDEL and calreticulin signal peptide
    Expression
    Mammalian
    Mutation
    S175G, D180Y, I167V and L15Q in GFP moiety and D…
    Promoter
    CMV
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    78118-plasmid-map-sequence-id-153361

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  15. pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A

    Plasmid
    #154773
    Purpose
    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Y125A in G1PylS, hybrid PylT with A41AA and C55A …
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154773-plasmid-map-sequence-id-310428

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