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pLL5.0-Anillin shRNA

Plasmid

#187270

Purpose

Lentiviral expression of shRNA targeting Anillin

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

shRNA for Anillin (ANLN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

U6

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2b-WT

Plasmid

#45698

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pTWIN1-His6-Ssp-Httex1-23Q

Plasmid

#84349

Purpose

Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Use

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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TK-GFP-Nluc-P2A-neo-TK

Plasmid

#134896

Purpose

Expresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Insert

TK-GFP-Nluc-P2A-Neo-TK

Use

Cryptosporidium expression

Tags

Expression

Mutation

Promoter

Cryptosporidium actin and enolase

Available since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG R2pH-LAMP1-3xFLAG

Plasmid

#157940

Purpose

expresses ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

R2pH-LAMP1-3xFLAG (Lamp1 Mouse)

Use

Tags

3XFLAG, mCherry, and pHluorin

Expression

Mammalian

Mutation

Promoter

Chick beta actin pomoter

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Cdx2

Plasmid

#128829

Purpose

dox-dependent expression of mouse Cdx2

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Cdx2 (Cdx2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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His6tev CBP HAT (1324-1700)

Plasmid

#99340

Purpose

bacterial expression of His tagged mouse CBP HAT domain (1324-1700)

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP HAT domain (1324-1700) (Crebbp Mouse)

Use

Tags

His6tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pET-pro-siRNA-V2-TP53

Plasmid

#111089

Purpose

pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.

Depositor

Linfeng HUANG

Article

Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

Insert

TP53 (TP53 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -2

Plasmid

#173888

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shSTING-Hsa

Plasmid

#128158

Purpose

Doxycyclin inducible shRNA knockdown of human STING gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shSTING Hsa (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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METTL3 shRNA 2 tet pLKO puro

Plasmid

#162984

Purpose

Tet-inducible shRNA targeting human METTL3 #2

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

methyltransferase like 3 (METTL3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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His6tev CBP BCHZ (1079-1751)

Plasmid

#99341

Purpose

Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)

Use

Tags

His6tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-7Q

Plasmid

#84347

Purpose

Expression of the human Huntingtin Exon1 protein containing 7Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Use

Tags

N-terminal His6-tagged intein Ssp

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Golgi-7

Plasmid

#58018

Purpose

Localization: Golgi Complex, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Golgi (B4GALT1 Human)

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pC-SUR-YR

Plasmid

#61768

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).

Depositor

Ken Haynes

Article

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

Sur gene
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Tags

Expression

Mutation

Promoter

Magnaporthe oryzae ILV promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pIE-TGFB2-H6

Plasmid

#128500

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (TGFB2 Human)

Use

Tags

His tag (6x)

Expression

Insect

Mutation

Promoter

IE1

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG_mRuby2_smFP OLLAS

Plasmid

#59761

Purpose

encodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

mRuby2_smFP_OLLAS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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Myc-E-Syt1

Plasmid

#66833

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt1 (ESYT1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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mEos3.2FPP

Plasmid

#203758

Purpose

Encodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

HRas c-term (HRAS Human)

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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RAPpGEX

Plasmid

#162241

Purpose

Expresses LDL-receptor associated protein (RAP), full length in mammalian cells

Depositor

Andreas Faissner

Article

Hennen et al J Biol Chem. 2013 Jun 7;288(23):16538-45

Insert

lrpap1 (Lrpap1 Mouse)

Use

Tags

GST-tag

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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