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Plasmid#74067PurposepAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecRDepositorInsertsgRNA pgi (NT)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-pyk_NT
Plasmid#74072PurposepAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecRDepositorInsertsgRNA pyk (NT)
UseCRISPRTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P2
Plasmid#194344PurposesgRNA position entry clone 2DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P1
Plasmid#194343PurposesgRNA position entry clone 1DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P3
Plasmid#194345PurposesgRNA position entry clone 3DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P4
Plasmid#194346PurposesgRNA position entry clone 4DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P5
Plasmid#194347PurposesgRNA position entry clone 5DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P6
Plasmid#194348PurposesgRNA position entry clone 6DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P7
Plasmid#194349PurposesgRNA position entry clone 7DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBFv-U6.2B
Plasmid#138401PurposegRNA expression vector; Can be combined with pBFv-U6.2 to generate double-gRNA expression vectorDepositorInsertU6-2
UseTagsExpressionInsectMutationPromoterU6-2Available sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJEC625
Plasmid#174368PurposeGolden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJF131B
Plasmid#113326PurposepTet-W108 scRNA.b2, pTet-RR2-sgRNADepositorInsertW108 scRNA_MS2 and sgRNA_RR2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAL-pgi_T
Plasmid#74066PurposepAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecRDepositorInsertsgRNA pgi (T)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-rfp_T
Plasmid#74075PurposepAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecRDepositorInsertsgRNA rfp (T)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-pyk_T
Plasmid#74070PurposepAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecRDepositorInsertsgRNA pyk (T)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-pck_T
Plasmid#74068PurposepAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecRDepositorInsertsgRNA pck (T)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgPal1 (p2Tol-U6-sgPal1-HygR)
Plasmid#71483PurposeExpresses palindromic sgRNA for use in self-cloning CRISPRDepositorInsertsgPal1
UseTagsExpressionMutationPromoterU6Available sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Guide-Generating Vector
Plasmid#125752PurposeGuide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assemblyDepositorInsertCys4-GFPdropout-SpCas9scaffold
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWT060e
Plasmid#107905PurposeW2m.0-3, W2m.3-2 plasmid. Contains U6-sgRNA B (CCR5) and is part of the CAMERA2m system for cellular recording in mammalian cellsDepositorInsertU6-sgRNA B (CCR5)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa VP64 Afp
Plasmid#99697PurposeExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAVDepositorInsertdCas9 and gRNA targeting Afp (Afp Synthetic)
UseAAV and CRISPRTagsVP64ExpressionMutationdead Cas9PromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only