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Plasmid#105522PurposeT7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryExpressionMutationPromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-mTrim21
Plasmid#105519PurposeT7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmEGFPExpressionMutationPromoterT7Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-UPF1-3'UTR
Plasmid#136036PurposeUPF1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (TTATTACCCAGAATAAGATGC)DepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-UPF1-CDS
Plasmid#136037PurposeUPF1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (AAGACACCTATTACACGAAGG)DepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
TPI-WT
Plasmid#130697PurposeExpresses TPI-Wildtype NMD reporterDepositorInsertTPI-Wildtype
UseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP GFP-TREX1
Plasmid#164245Purposeretroviral plasmid for GFP-TREX1DepositorInsertTREX1 (TREX1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-GFP-RPA70
Plasmid#164231Purposeretroviral plasmid for GFP-RPA70DepositorInsertRPA70 (RPA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-PLBD2-6xHis
Plasmid#213606PurposeExpresses PLBD2-6xHis in mammalian cellsDepositorInsertphospholipase B domain containing 2 (PLBD2 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ 3xFLAG-TREX1
Plasmid#164243Purposeretroviral plasmid for 3xFLAG-TREX1DepositorInsertTREX1 (TREX1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hSOX2
Plasmid#17226DepositorInsertSRY (sex determining region Y)-box 2 (SOX2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE2-blast
Plasmid#208034PurposeEnables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE2-puro
Plasmid#208032PurposeEnables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2000
Plasmid#139885PurposeExpression plasmid for LetAB with 2 ring (rings17) LetB (without TM region) mutantDepositorInsertLetB
UseTagsExpressionBacterialMutationLetB(43-877; delta161-747)-6xHisPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-ciBAR1
Plasmid#200440PurposeExpresses Flag-tagged human ciBAR1 in mammalian cellsDepositorInsertciBAR1
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-ciBAR2
Plasmid#200441PurposeExpresses Flag-tagged human ciBAR2 in mammalian cellsDepositorInsertciBAR2
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CD44U-c010
Plasmid#175453PurposeProtein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649.DepositorInsertCD44 (CD44 Human)
UseTagsHis6ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL29
Plasmid#174063PurposeExpresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter.DepositorInsert2x43-8 ZFO-1x97-4 ZFO pCyc mCherry tCyc
UseTagsFLAGExpressionYeastMutationPromoterpCyc1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA
Plasmid#140224PurposeConstitutive PGK driven expression of intein with miniVPR for 3-way split activatorDepositorInsertC-intein with miniVPR
UseTagsmCherryExpressionMammalianMutationPromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp YFP-PIF-SOS2cat
Plasmid#50851PurposeOptogenetic Ras activation system; cytoplasmic componentDepositorInsertsUseLentiviralTagsYFPExpressionMutationresidues 566–1049PromoterAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp BFP-PIF-SOS2cat
Plasmid#50850PurposeOptogenetic Ras activation system; cytoplasmic componentDepositorInsertsUseLentiviralTagsTagBFPExpressionMutationresidues 566–1049PromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only