-
Plasmid#45259DepositorInsertARPM1 (ACTRT3 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
p5E-EFS (EF1a core) (JDW 1319)
Plasmid#224474PurposeGateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.DepositorInsertEFS promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Luciferase-P2A-H2A-mCherry (JDW 926)
Plasmid#224488PurposeGateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)DepositorInsertLuciferase-P2A-H2A-mCherry
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME Actin Vhh Halo Tag (JDW 1223)
Plasmid#224498PurposeGateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)DepositorInsertActin-Vhh-Halo-Tag
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hCB2R
Plasmid#179322PurposeExpress human cannabinoid 2 receptor (GFP tagged) in mammalian cellsDepositorInserthuman cannabinoid 2 receptor (CNR2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)
Plasmid#224505PurposeGateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expressionDepositorInsertLexA-mODC-LexOP-35SCaMV
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-LexA-mODC-LexOP-c-Fos (JDW 1359)
Plasmid#224506PurposeGateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression constructDepositorInsertLexA-oMDC-LexOP-c-Fos
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)
Plasmid#224512PurposeA Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)DepositorInsert3xHA-BirA-T2A-mCherry-Caax
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)
Plasmid#224522PurposeA Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassetteDepositorInsertlox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1:mTRESK(S116C)
Plasmid#224772PurposeIn vitro transcription for Oocyte expression of mTRESK(S116C)DepositorInsertPotassium channel subfamily member 18 (Kcnk18 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS116CPromoterT7Available sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:hTRAAK(S118C/Q258K)
Plasmid#224764PurposeIn vitro transcription for Oocyte expression of hTRAAK(S118C/Q258K)DepositorInsertPotassium channel subfamily member 4 (KCNK4 Human)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS118C/Q258KPromoterT7Available sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1
Plasmid#224773PurposeMammalian expression vector for generating mouse TREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1(S131C)
Plasmid#224774PurposeMammalian expression vector for generating mouse TREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationS131CPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(S131C)
Plasmid#224744PurposeIn vitro transcription for Oocyte expression of mTREK-1(S131C)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131CPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1 (C159A/C219A)
Plasmid#224751PurposeIn vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationC159A/C219APromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTRAAK(S93C/Q233K)
Plasmid#224768PurposeIn vitro transcription for Oocyte expression of mTRAAK(S93C/Q233K)DepositorInsertPotassium channel subfamily member 4 (Kcnk4 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS93C / Q233KPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(S131A)
Plasmid#224745PurposeIn vitro transcription for Oocyte expression of mTREK-1(S131A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131APromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTRAAK(Q233K)
Plasmid#224767PurposeIn vitro transcription for Oocyte expression of mTRAAK(Q233K)DepositorInsertPotassium channel subfamily member 4 (Kcnk4 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationQ233KPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 TREK-2(S156C)
Plasmid#224770PurposeIn vitro transcription for Oocyte expression of 1 TREK-2(S156C)DepositorInsertPotassium channel subfamily member 10 (Kcnk10 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS156CPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(G171F)
Plasmid#224747PurposeIn vitro transcription for Oocyte expression of mTREK-1(G171F)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationG171FPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only