Addgene

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pBabe_puro_DEST_Flag_ARPM1

Plasmid

#45259

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

ARPM1 (ACTRT3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
p5E-EFS (EF1a core) (JDW 1319)

Plasmid

#224474

Purpose

Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

EFS promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Luciferase-P2A-H2A-mCherry (JDW 926)

Plasmid

#224488

Purpose

Gateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Luciferase-P2A-H2A-mCherry

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME Actin Vhh Halo Tag (JDW 1223)

Plasmid

#224498

Purpose

Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Actin-Vhh-Halo-Tag

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-hCB2R

Plasmid

#179322

Purpose

Express human cannabinoid 2 receptor (GFP tagged) in mammalian cells

Depositor

Christian Gruber

Article

Tomašević et al J Biol Chem. 2024 Jun;300(6):107330. doi

Insert

human cannabinoid 2 receptor (CNR2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

Plasmid

#224505

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

LexA-mODC-LexOP-35SCaMV

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

Plasmid

#224506

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

LexA-oMDC-LexOP-c-Fos

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

Plasmid

#224512

Purpose

A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

3xHA-BirA-T2A-mCherry-Caax

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

Plasmid

#224522

Purpose

A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1:mTRESK(S116C)

Plasmid

#224772

Purpose

In vitro transcription for Oocyte expression of mTRESK(S116C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 18 (Kcnk18 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S116C

Promoter

T7

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(S118C/Q258K)

Plasmid

#224764

Purpose

In vitro transcription for Oocyte expression of hTRAAK(S118C/Q258K)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S118C/Q258K

Promoter

T7

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1

Plasmid

#224773

Purpose

Mammalian expression vector for generating mouse TREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(S131C)

Plasmid

#224774

Purpose

Mammalian expression vector for generating mouse TREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

S131C

Promoter

CMV

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C)

Plasmid

#224744

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (C159A/C219A)

Plasmid

#224751

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

C159A/C219A

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTRAAK(S93C/Q233K)

Plasmid

#224768

Purpose

In vitro transcription for Oocyte expression of mTRAAK(S93C/Q233K)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (Kcnk4 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S93C / Q233K

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131A)

Plasmid

#224745

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131A

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTRAAK(Q233K)

Plasmid

#224767

Purpose

In vitro transcription for Oocyte expression of mTRAAK(Q233K)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (Kcnk4 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

Q233K

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TREK-2(S156C)

Plasmid

#224770

Purpose

In vitro transcription for Oocyte expression of 1 TREK-2(S156C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 10 (Kcnk10 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S156C

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(G171F)

Plasmid

#224747

Purpose

In vitro transcription for Oocyte expression of mTREK-1(G171F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

G171F

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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