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Plasmid#85768Purposebacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusionDepositorInsertUSPL1, catalytic domain (aa 212-514) (USPL1 Human)
UseTagsGST and HisExpressionBacterialMutationcatalytic domain (aa 212-514)PromoterT7-lacAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETNb-cALFA
Plasmid#159989PurposeFor the protein expression with ALFA tag at C-terminus in E. coliDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a.Tat(48-57)-EGFP
Plasmid#165040PurposeExpression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)DepositorInsertEGFP
UseTags6XHis and Tat(48-57)ExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(cd226)
Plasmid#177900PurposeBacterial expression of his10-tagged human cd226 Intracellular DomainDepositorInsertCD226 (aa 288-336 only) (CD226 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABE
Plasmid#73438PurposeProdeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.DepositorInsertPG6-vioABE
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPG6 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_STRAW
Plasmid#204240PurposeBacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_STRAW (mdh Streptomyces avermitilis)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI
Plasmid#50723Purposeoverexpression of human TPI1 in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgo
Plasmid#210191PurposeBacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
UseTags6x His tag and SUMO tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-mtPheRS
Plasmid#31080DepositorInsertMature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)
UseTagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Nt …PromoterT7Available sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28GST-LIC
Plasmid#26101PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTags6XHis, GST, and Thrombin CLeavage SiteExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-2(KW)2-mCherry
Plasmid#112738PurposeExpress 2(KW)2-mCherry in E. coliDepositorInsert2(KW)2-mCherry
UseTags6xHisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET14-GFP15_Encap
Plasmid#86407PurposeExpresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 15aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CLTC_Clathrin propeller
Plasmid#178471PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCLTC_Clathrin propeller (CLTC Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL1-YPet
Plasmid#102633PurposeIn vitro expression of YPet from the T7 promoterDepositorInsertYPet
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30a-FluA-NP
Plasmid#165092Purposeprotein expression in E. coliDepositorInsertInfluenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET15-EF1p_mFAP2b
Plasmid#159469PurposeEncodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF1p_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-2(KW)2-eGFP
Plasmid#112736PurposeExpress 2(KW)2-eGFP in E. coliDepositorInsert2(KW)2-eGFP
UseTags6xHisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15-EF4n_mFAP2b
Plasmid#159464PurposeEncodes for EF4n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF4n_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b-CPDSacI
Plasmid#38255DepositorTypeEmpty backboneUseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL + STS
Plasmid#139790Purposeexpression of biosynthetic enzymes 4CL and STSDepositorInserts4CL
STS
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only