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Plasmid#133962PurposeCMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcriptionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7 RNA POL
Plasmid#138352PurposeExpressing T7 RNA Polymerase in mammalian cellsDepositorInsertT7 RNA POL
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RR-ABE8e-V106W
Plasmid#193656PurposeExpression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-TadA*8e-V106W, EGFP
UseTagsExpressionMammalianMutationdLbCas12a-G532R/K595R, TadA*8e-V106WPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4-SsAPOBEC3B
Plasmid#138343PurposeMammalian expression plasmid for BE4-SsAPOBEC3BDepositorInsertBE4-SsAPOBEC3B
UseSynthetic BiologyTagsBP-NLSExpressionMutationPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-ABE7
Plasmid#193644PurposeExpression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-TadA-TadA*7.10, EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam
Plasmid#16440PurposeContains two independent transcription units--a CMV promoter/enhancer upstream of a MCS and a HSV thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-sadCas9-VP64
Plasmid#115790PurposeAn AAV vector expressing S. aureus dCas9 fused to VP64.DepositorInsertSa-dCas9
UseAAVTagsNLS and NLS-VP64ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-GABARAPL1 WT
Plasmid#123100PurposeExpresses 3xFLAG-GABARAPL1 wild-type in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-C-BE4max
Plasmid#183679PurposeExpresses eNme2-C-BE4 in mammalian cellsDepositorInsertrAPOBEC1-neNme2-C-2xUGI
UseCRISPRTagsExpressionMammalianMutationNme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 TBRI-His
Plasmid#19161DepositorInserttransforming growth factor, beta receptor 1 (TGFBR1 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-15F11-HA-mEGFP
Plasmid#129590PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-M3nls
Plasmid#155366PurposeTargeted m6A RNA methylation in mammalian cellsDepositorInsertdCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
UseCRISPRTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133APromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PARP1-3xFlag-WT
Plasmid#111575PurposeExpresses full length PARP1 in mammalian cellsDepositorInsertPARP1 (PARP1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Vamp2-pHluorin
Plasmid#190151PurposeExpresses (pH-sensitive) super ecliptic pHluorin-tagged mouse/rat Vamp2 for imaging exocytosisDepositorInsertVamp2 (Vamp2 Mouse)
UseAAVTagssuperecliptic pHluorinExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-SpRY
Plasmid#159979PurposePrime editing in mammalian cellsDepositorInsertPE2-SpRY
UseTagsBpSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMVβ-p300-myc
Plasmid#30489PurposeMammalian expression of human p300 with myc tagDepositorInsertp300 (EP300 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cas9-NRCH
Plasmid#136926PurposeExpresses Cas9-NRCH in mammalian cellsDepositorInsertCas9-NRCH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-M3M14nes
Plasmid#155367PurposeTargeted m6A RNA methylation in mammalian cellsDepositorInsertUseCRISPRTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133APromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-lyso-pHoenix
Plasmid#70112PurposeExpression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomesDepositorInsertlyso-pHoenix (Cd63 Mouse, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)
UseTagsArch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT PINK1 C-myc
Plasmid#13314DepositorInsertPINK1 (PINK1 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only