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Plasmid#182836PurposeSaccharomyces cerevisiae inducible CRISPRai vector - NatR markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only
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pLV hUbC-Cas9-T2A-GFP
Plasmid#53190Purpose3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFPDepositorInserthumanized Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationPromoterhUbCAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLSSmOrange-mKate2
Plasmid#99868PurposeMeasure FRET between LSSmOrange and mKate2DepositorInsertLSSmOrange
UseTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9 KRAB-T2A-GFP
Plasmid#67620PurposeCo-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFPDepositorInserthumanized dead Cas9 KRAB T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_[Cox8a]x2_HaloTag9
Plasmid#175539PurposeExpression of HaloTag9 at the inner membrane of mitochondria in mammalian cellsDepositorInsertCox8a-HaloTag9
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE048-SiT-Cas12a
Plasmid#128124PurposeExpress both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ140
Plasmid#99895PurposeEmpty vector with attL5-attL2 recombination sites for Gateway CloningDepositorTypeEmpty backboneUseCRISPR and TALENTagsExpressionMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMPRAv3:∆luc:∆xbaI
Plasmid#109035PurposeMPRA v3 empty backboneDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoternoneAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS3799-gRNA-Csy4-template
Plasmid#182827PurposeSpCas9 gRNA-Csy4 templateDepositorInsertSpCas9 gRNA scaffold + Csy4 sequence
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS5321-CRISPRai-Markerless
Plasmid#182828PurposeSaccharomyces cerevisiae inducible CRISPRai vector - markerlessDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI118-SiT-Cas12a-[Ind]
Plasmid#128406PurposeInducible expression of both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWS5396-CRISPRai-KanR
Plasmid#182835PurposeSaccharomyces cerevisiae inducible CRISPRai vector - KanR markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCE046-SiT-Cas12a-[Repr]
Plasmid#128132PurposeExpress both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcriptDepositorInsertAsCas12a-[Repr]-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWS5398-CRISPRai-HygR
Plasmid#182837PurposeSaccharomyces cerevisiae inducible CRISPRai vector - HygR markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCE059-SiT-Cas12a-[Activ]
Plasmid#128136PurposeExpress both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcriptDepositorInsertAsCas12a-[Activ]-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWS5390-CRISPRai-URA3
Plasmid#182829PurposeSaccharomyces cerevisiae inducible CRISPRai vector - URA3 markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
2xP53_RE::FLuc
Plasmid#178316PurposeP53 Firefly luciferase reporterDepositorInsertP53 FLuc Reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS5394-CRISPRai-MET17
Plasmid#182833PurposeSaccharomyces cerevisiae inducible CRISPRai vector - MET17 markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS5392-CRISPRai-HIS3
Plasmid#182831PurposeSaccharomyces cerevisiae inducible CRISPRai vector - HIS3 markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS5393-CRISPRai-TRP1
Plasmid#182832PurposeSaccharomyces cerevisiae inducible CRISPRai vector - TRP1 markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only