Addgene

We narrowed to 14,719 results for: grna

Showing: 721 - 740 of 14719 results

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LC3B sgRNA

Plasmid

#207556

Purpose

pX330 expressing Cas9 and a sgRNA targeting the LC3B locus

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

CACTGAATACCAGCGCCTAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA_MET_uORF1

Plasmid

#216537

Purpose

gRNA and template for MET 5'UTR uORF1 mutation

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 7 Jan 2025. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF1 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA_MET_uORF2

Plasmid

#216538

Purpose

gRNA and template for MET 5'UTR uORF2 mutation

Depositor

Paolo Comoglio

Article

Cerqua et al EMBO J. 7 Jan 2025. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF2 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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RhoA gRNA #1

Plasmid

#198718

Purpose

Cas9-mediated knockout of RhoA in mammalian cells #1

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoA (Rhoa Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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RhoA gRNA #3

Plasmid

#198720

Purpose

Cas9-mediated knockout of RhoA in mammalian cells #3

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoA (Rhoa Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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RhoB gRNA #1

Plasmid

#198721

Purpose

Cas9-mediated knockout of RhoB in mammalian cells #1

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoB (Rhob Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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RhoB gRNA #2

Plasmid

#198722

Purpose

Cas9-mediated knockout of RhoB in mammalian cells #2

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoB (Rhob Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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RhoB gRNA #3

Plasmid

#198723

Purpose

Cas9-mediated knockout of RhoB in mammalian cells #3

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoB (Rhob Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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RhoC gRNA #1

Plasmid

#198724

Purpose

Cas9-mediated knockout of RhoC in mammalian cells #1

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoC (Rhoc Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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RhoC gRNA #2

Plasmid

#198725

Purpose

Cas9-mediated knockout of RhoC in mammalian cells #2

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoC (Rhoc Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RhoC gRNA #3

Plasmid

#198726

Purpose

Cas9-mediated knockout of RhoC in mammalian cells #3

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoC (Rhoc Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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p103DestTol2pA2-4xU6sgRNA

Plasmid

#227778

Purpose

Destination vector with U6 driven 4 sgRNAs

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

4xU6:sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCF142_U6-sgRNA

Plasmid

#225960

Purpose

U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

Depositor

Christof Fellmann

Article

Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

Insert

U6-sgRNA (recipient)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA1_Cas9-hGem_Smc4

Plasmid

#217667

Purpose

expresses Cas9-hGem and guideRNA for tagging of SMC4

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA1 targeting SMC4 C-terminal (SMC4 Human)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 NT sgRNA3

Plasmid

#164929

Purpose

Expresses Non-targeting sgRNA3 control in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

Non-targeting sgRNA3 (verified for knockout)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 NT sgRNA5

Plasmid

#164930

Purpose

Expresses Non-targeting sgRNA5 control in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

Non-targeting sgRNA5 (verified for knockout)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1_Cas9-hGem_gRNA2

Plasmid

#217659

Purpose

expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA2 targeting the human AAVS1 safe harbour locus

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria_speG_M15

Plasmid

#220488

Purpose

inducible CRISPRi

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

gspeG

Use

CRISPR and RNAi

Tags

Expression

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria_speG_E23

Plasmid

#220489

Purpose

inducible CRISPRi

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

gspeG

Use

CRISPR and RNAi

Tags

Expression

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #1

Plasmid

#207105

Purpose

pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CGCTATCAAGATTTATACCT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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