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Plasmid#117475PurposeGateway Vector for Fbxo8 without stop codonDepositorInsertFbxo8 (FBXO8 Human)
UseGateway shuttling vectorTagsExpressionMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
R777-E019 Hs.CCND2
Plasmid#70303PurposeGateway ORF clone of human CCND2 [NM_001759.1] with stop codon (for native or N-terminal fusions)DepositorInsertCCND2 (CCND2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E294 Hs.RPS6KA3-nostop
Plasmid#70578PurposeGateway ORF clone of human RPS6KA3 [NM_004586.2] without stop codon (for C-terminal fusions)DepositorInsertRPS6KA3 (RPS6KA3 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E292 Hs.RPS6KA2-nostop
Plasmid#70576PurposeGateway ORF clone of human RPS6KA2 [NM_021135.4] without stop codon (for C-terminal fusions)DepositorInsertRPS6KA2 (RPS6KA2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-deltaUBA-short-UBASH3A-Ctag
Plasmid#195397PurposeHuman short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for C-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationUBASH3A mutant lacking the UBA domainPromoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-deltaUBA-short-UBASH3A-Ntag
Plasmid#195398PurposeHuman short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationUBASH3A mutant lacking the UBA domainPromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-nrip1b
Plasmid#192732PurposeGateway entry vector encoding zebrafish nrip1bDepositorInsertnrip1b (nrip1b Zebrafish)
UseGateway entry vectorTagsExpressionMutationL661P (rs511527097); S720P; A801T; K862E; A863P; …PromoterNoneAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
R777-E096 Hs.FNTB-nostop
Plasmid#70380PurposeGateway ORF clone of human FNTB [NM_002028.3] without stop codon (for C-terminal fusions)DepositorInsertFNTB (FNTB Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E022 Hs.CCND3-nostop
Plasmid#70306PurposeGateway ORF clone of human CCND3 [NM_001760.4] without stop codon (for C-terminal fusions)DepositorInsertCCND3 (CCND3 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-mCherry
Plasmid#123221PurposeN-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-EGFP
Plasmid#122848PurposeN-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-3xFLAG
Plasmid#123223PurposeN-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-polysite
Plasmid#122843PurposeGateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.DepositorTypeEmpty backboneUseGateway destinationTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
1455_pDEST_miniTol2_R4-R2_MCS
Plasmid#171794PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a Multiple Cloning Site for selection marker insertion.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEpic
Plasmid#84372Purpose3rd-generation lentiviral destination vector for gateway cloning with puromycin resistance.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME Actin Vhh mCherry (JDW 1240)
Plasmid#224499PurposeGateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)DepositorInsertActin-Vhh-mCherry
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-DreERT2 (JDW 1344)
Plasmid#224523PurposeA Gateway compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activityDepositorInsertDreERT2
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-3x-FLAG-Ubiquitin (JDW 1098)
Plasmid#224513PurposeA Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation statusDepositorInsert3x-FLAG-Ubiquitin
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-EGFP
Plasmid#48349PurposeContributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEGFP
UseGateway entry vectorTagsExpressionMutationAminoacid 40 is valine in our sequence, while it …PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-V5-6xHis
Plasmid#48347PurposeContributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertV5 and 6xHis epitope tags cassette
UseGateway entry cloneTagsExpressionMutationContains a stop codon at the end of the epitope t…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only