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Plasmid#219762PurposeMoClo-compatible Level 1 for expression HmS in plants (N.benthamiana, BY2 cells)DepositorInsertHmS
UseTagsExpressionPlantMutationPromoterp35s_0.4kb - 5'UTR TMV omegaAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7191
Plasmid#219764PurposeMoClo-compatible Level M for expression PpASCL in plants (N.benthamiana, BY2 cells)DepositorInsertPpASCL
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7181
Plasmid#219766PurposeVector for expression HmS in yeast (P.pastoris)DepositorInsertHmS
UseTagsExpressionYeastMutationPromoterpGAPAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7182
Plasmid#219767PurposeVector for expression PpASCL in yeast (P.pastoris)DepositorInsertPpASCL
UseTagsExpressionYeastMutationPromoterpGAPAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7188
Plasmid#219768PurposeVector for expression Pv4CL1 in yeast (P.pastoris)DepositorInsertPv4CL1
UseTagsExpressionYeastMutationPromoterpGAPAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX030
Plasmid#167154PurposeMoClo-compatible Level 1 (position 5) vector encoding Neonothopanus nambi caffeoylpiruvate hydrolase nnCPH under control of 0.4 kb 35S promoter, for expression in plantsDepositorInsertp35s-nnCPH-ocsT
UseTagsExpressionPlantMutationPromoterp35sAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX026
Plasmid#167153PurposeMoClo-compatible Level 1 (position 4) vector encoding Neonothopanus nambi luciferase nnLuz with C-terminal His-tag under control of 0.4 kb 35S promoter, for expression in plantsDepositorInsertp35s-nnLuz-ocsT
UseLuciferaseTagsHis6-tagExpressionPlantMutationPromoterp35sAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB120
Plasmid#167150PurposeMoClo-compatible Level 1 (position 1) vector encoding Kanamycin resistance cassette, for expression in plantsDepositorInsertpNOS-nptII-ocsT
UseTagsExpressionPlantMutationPromoterpNOSAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX022
Plasmid#167148PurposeMoClo-compatible Level 0-CDS2 promoterless vector encoding C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamianaDepositorInsertC-part of fungal hispidin synthase, nnHispS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX021
Plasmid#167147PurposeMoClo-compatible Level 0-SP promoterless vector encoding N-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamianaDepositorInsertN-part of fungal hispidin synthase, nnHispS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG2b-srt-his
Plasmid#124804PurposeHDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.DepositorInsertMurine IgG2b
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG2a-srt-his
Plasmid#124803PurposeHDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.DepositorInsertMurine IgG2a
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmEos2-N1 GBP (GBP-mEos2)
Plasmid#162876PurposeExpression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization MicroscopyDepositorInsertGFP Binding Protein tagged with mEos2
UseTagsmEos2ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX459-gR2A_Hinge
Plasmid#124811PurposeVector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.DepositorInsertCas9 – gRNA_R2A_hinge
UseCRISPRTags3XFLAG and GFPExpressionBacterial and MammalianMutationR166H in PuroR (please see depositors comment bel…PromoterpUCAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG1-srt-his
Plasmid#124802PurposeHDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.DepositorInsertMurine IgG1
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationPromoter-Available sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mA-srt-his
Plasmid#124806PurposeHDR template to knock-in mouse IgA Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgA producing cell lines.DepositorInsertMurine IgA
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG3-srt-his
Plasmid#124805PurposeHDR template to knock-in mouse IgG3 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG3 producing cell lines.DepositorInsertMurine IgG3
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-dL5-Her1
Plasmid#73215PurposeBacterial expression vector for dL5(E52D)-Z1907 affibody fusion protein (FA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)DepositorInsertEGFR (EGFR Schistosoma japonicum, Synthetic, Human)
UseTags10XHis-GST-HRV and The dL5 FAP and Her1 affibody …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-control
Plasmid#213788PurposeCIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-Calm3-U6-control gRNA
UseLentiviralTagsGFPExpressionMammalianMutationPromoterSyn1, U6Available sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-Gas5
Plasmid#213172PurposeCIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-Calm3-U6-Gas5 gRNA
UseLentiviralTagsGFPExpressionMammalianMutationPromoterSyn1, U6Available sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only