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Plasmid#179798PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-sfGFP
Plasmid#179782PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertsfGFP
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-blast-3Ty:mNG:3Ty
Plasmid#179783PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-eYFP
Plasmid#179784PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInserteYFP
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
LZ3 dFE
Plasmid#190144PurposeExpresses LZ3Cas9-T4pol (LZ3 dFE)DepositorInsertLZ3Cas9-T4pol (LZ3 dFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HiFi dFE
Plasmid#190142PurposeExpresses HiFiCas9-T4pol (HiFi dFE)DepositorInsertHiFi Cas9-T4pol (HiFi dFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationSpCas9 R691A mutant, Codon Optimized for mammalia…PromoterCBhAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH020
Plasmid#186633Purposecontains homology arms for the pilA (NIES39_C03030) locus of Arthrospira platensisDepositorArticleTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH021
Plasmid#186634Purposecontains homology arms for the NSI (NIES39_Q01230) locus of Arthrospira platensisDepositorArticleTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH022
Plasmid#186635Purposecontains homology arms for the oscillin (NIES39_A01430) locus of Arthrospira platensisDepositorArticleTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH036
Plasmid#186637PurposeaadA.co between homology arms for NSIDepositorArticleInsertaadA.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH043
Plasmid#186638PurposeaadA.co between homology arms for oscillinDepositorArticleInsertaadA.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector
Plasmid#194726PurposeCAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vectorDepositorArticleInsertAsCpf1(D908A)
UseCRISPRTagsExpressionMammalianMutationD908APromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX458-MAFB-sg
Plasmid#194720Purposepx458 with guide RNA that target hMAFBDepositorArticleInsertCas9 (MAFB Streptococcus pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_mdHUR-Halo
Plasmid#175312PurposeProduction of HaloTag fusion proteinDepositorInsertHUR
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS33
Plasmid#171202Purposebacterial experssion of a GST-tagged MEG-3 C-terminus with mutations in the HMG-like motifDepositorInsertmeg-3 (meg-3 Nematode)
UseTagsGSTExpressionBacterialMutationaa545-862, F705A,Y708A,Y713A, N725APromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB252
Plasmid#170035PurposeEncodes LacI-controlled expression of surface-displayed K6L9 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
K6L9
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB256
Plasmid#170039PurposeEncodes LacI-controlled expression of surface-displayed Bac2a and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Bac2a
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAP21
Plasmid#171207Purposebacterial expression for HIS purification of the MEG-3 C-terminus with mutations in the HMG-like motifDepositorInsertmeg-3 (meg-3 Nematode)
UseTags8xHISExpressionBacterialMutationaa545-862, F705A,Y708A,Y713A, N725APromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW004
Plasmid#131805PurposeVector constitutively expressing Zea mays FNR and Z. mays SIR with an insertion following BCD13 and preceeding the zmFNR gene deleted 'GGAGAGCATTAGGTCTCG'DepositorArticleInsertZea mays FNR and Z. mays SIR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG09-OR2-OR1-Pr-sigma54
Plasmid#102466PurposeExpresses sigma 54 protein in E. coli. The expression is controlled by a OR2-OR1-Pr promoter.DepositorArticleInsertsigma 54 (rpoN )
UseTagsExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only