Addgene

We narrowed to 2,242 results for: PRK

Showing: 721 - 740 of 2242 results

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pRK5-myc-Miro1 Δ593-618

Plasmid

#47895

Purpose

Expresses myc tagged Miro1 lacking transmembrane domain

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro1 Δ593-618 (RHOT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Δ593-618, lacks transmembrane domain

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-mEGFP-HMGB1-MUT-IDR

Plasmid

#194551

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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prk5-MFSD12-TEV-3xFLAG-3xHA

Plasmid

#149504

Purpose

Transient expression of human MFSD12 with recoded nucleotide sequence.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

MFSD12 (MFSD12 Human)

Use

Transient expression

Tags

TEV-3XHA-3XFLAG

Expression

Mammalian

Mutation

Codon Optimized Sequence

Promoter

CMV

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-F5A-E389-deltaCT

Plasmid

#8990

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

F5A, T389E, stop codon at amino acid 402

Promoter

Available since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
pRK5-mEGFP-HMGB1-MUT-IDR2

Plasmid

#194552

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 E208K/E328K

Plasmid

#47900

Purpose

Expresses myc tagged Miro2 V208K/E328K mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 E208K/E328K (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

E208K/E328K, abolishes calcium binding

Promoter

CMV

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-mEGFP-HMGB1-MUT-RtoK

Plasmid

#194559

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R > K" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

R > K substitutions after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-KtoR

Plasmid

#194560

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "K > R" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K > R substitutions after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PRKAR2A

Plasmid

#20601

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKAR2A (PRKAR2A Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PRKACB

Plasmid

#20596

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKACB (PRKACB Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PRKAG2

Plasmid

#20656

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKAG2 (PRKAG2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2 Δ593-618

Plasmid

#47901

Purpose

Expresses myc tagged Miro2 lacking transmembrane domain

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 Δ593-618 (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Δ593-618, lacks transmembrane domain. Also see De…

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PRKCZ

Plasmid

#20607

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKCZ (PRKCZ Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pRK-flag-GFP 1-10 tetra Cys

Plasmid

#78589

Purpose

Expression flag-GFP 1-10 tetra Cys in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-GFP 1-10 tetra Cys

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 494-1318

Plasmid

#78587

Purpose

Expression flag-USP19 494-1318 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1318 (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PRKACG

Plasmid

#20597

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKACG (PRKACG Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PRKRA

Plasmid

#20608

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKRA (PRKRA Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
HA GST F5A deltaCT S6K1 pRK5

Plasmid

#8467

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

f5a delta CT p85 s6k1 (Rps6kb1 Rat)

Use

Tags

ha/gst

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 494-1290

Plasmid

#78580

Purpose

Expression flag-USP19 494-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1290 (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493 and mutated 1291 residue…

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-SZT2 2241-end

Plasmid

#87039

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AA 2241-end

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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