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Plasmid#129296PurposeExpresses 3xFLAG-ATG3 WT in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiCHATe27_Gq_dTomato
Plasmid#213832PurposeAAV construct expressing HA-hM3Dq-dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_Gq_dTomatoDepositorInsertHA-GqDREADD-dTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF3297
Plasmid#144773PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHIF1A (HIF1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS-mCherry
Plasmid#135457PurposeMammalian mCherry reporter to read out FLARE and SPARK activationDepositorInsertmCherry
UseTagsmCherryExpressionMammalianMutationPromoterUASAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterA. nidulans gpdA promoter with Hammerhead ribozym…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαs-LgB113
Plasmid#134362PurposeNanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.DepositorInsertGαs-LgB113 (GNAS Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_dSV40-Aurora A-GFP
Plasmid#67924PurposeExpresses Aurora A-GFP in mammalian cellsDepositorInsertGFP-Aurora A (AURKA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon DREADD Gi-mCherry
Plasmid#177672PurposeExpresses DREADD Gi in Cre and Flp expressing cellsDepositorHas ServiceAAV8InsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoternEFAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPROEX-TwStrp-FTO
Plasmid#190265PurposeExpresses FTO in bacterial cells, with two purification tags: His and Twin-Streptag.DepositorInsertFTO (FTO Human)
UseTags6xHis and Twin-StreptagExpressionBacterialMutationPromoterTrcAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX304-Flag-APEX2-NES
Plasmid#92158PurposeCytosolic APEX2 constructDepositorInsertFlag-APEX2-NES
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
shp53 pLKO.1 puro
Plasmid#19119Purpose3rd gen lentiviral vector for knocking down p53 gene expressionDepositorInsertsmall hairpin RNA against tumor protein p53 (TP53 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPGC1a_Proximal Promoter-FLuc2P
Plasmid#154259PurposeDestabilized firefly luciferase reporter containing the Human PGC-1alpha Proximal Promoter from -500 to +52.DepositorInsertPGC-1a Proximal Promoter (PPARGC1A Human)
UseTagsExpressionMammalianMutationPromoterHuman PGC-1alpha Proximal PromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRL056 pDESTattB_exorh:EGFP
Plasmid#213510PurposeattB-containing pDEST plasmid, for use in Multisite Gateway reactionDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx
Plasmid#36944DepositorInsertThioredoxin 1 (TXN Human)
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-neo-PP1-Alpha
Plasmid#171267PurposeMammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunitDepositorInsert3x-Flag-tagged human PP1 alpha catalytic subunit (PPP1CA Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαq-LgB97
Plasmid#134360PurposeNanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.DepositorInsertGαq-LgB97 (GNAQ Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 PIK3R1
Plasmid#17743DepositorInsertPIK3R1 (PIK3R1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pVH21-Tier1-PhEF1a
Plasmid#169561PurposeTier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Myo10WT
Plasmid#139780PurposeDetecting Myo10 by fluorescence microscopyDepositorInsertMyosin X (MYO10 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS1019_Tier3(PB)
Plasmid#169649PurposeTier-3 vector for stable integration of up to three cassettes via piggyBac transposase (ITR-A1::A2::A3-ITR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only