Addgene

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pDONR223-SRPK2

Plasmid

#23766

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SRPK2 (SRPK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

1MM

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/DEST/hTfR-HA

Plasmid

#69610

Purpose

human transferrin receptor with an HA tag fused at the C-terminus

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

human transferrin receptor (TFRC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCB591

Plasmid

#141096

Purpose

E. coli-Lactobacilli shuttle vector for recombineering template cloning

Depositor

Chase Beisel

Article

Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

Plasmid

#67981

Purpose

Cas9 activity reporter (control) with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_DA2m

Plasmid

#140553

Purpose

Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

The 2nd generation DA sensor GRAB_DA2m (medium affinity)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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AnkyrinB-2XHA

Plasmid

#31057

Depositor

Vann Bennett

Article

Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

Insert

Ankyrin B (ANK2 Human)

Use

Tags

2XHA

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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Halo-KDEL

Plasmid

#124316

Purpose

Expresses ER specific HaloTag protein

Depositor

Jin Wang

Article

Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

Insert

Halo tag

Use

Tags

KDEL Seq

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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Red cGull

Plasmid

#191853

Purpose

Expresses biosensor for cGMP in mammalian cells

Depositor

Takashi Tsuboi

Article

Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

Insert

Red cGull

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-CUL5

Plasmid

#19895

Depositor

Yue Xiong

Article

Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Insert

cullin 5 (CUL5 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pFB2G

Plasmid

#195290

Purpose

Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mTagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pECJ3

Plasmid

#75465

Purpose

Toggle switch plasmid containing lacI-pdt3

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

lacI-pdt3

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLtetO

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pKD3_mCherry

Plasmid

#187385

Purpose

To chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT

Depositor

Roi Avraham

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eNpHR 3.0-EYFP

Plasmid

#26972

Purpose

AAV expression of eNpHR 3.0 fused to EYFP driven by humanized Synapsin promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Has Service

AAV5

Insert

eNpHR 3.0

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Trafficking Signal (TS) ER Export Signal

Promoter

hSyn

Available since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_IRES_mC_pGK_tBFP

Plasmid

#79123

Purpose

Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 WT

Plasmid

#121446

Purpose

expression of huamn PD-L1 wildtype in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTlpA39-Wasabi

Plasmid

#86116

Purpose

mWasabi reporter gene gated by TlpA39

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA39
mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

D341A, D135V, A217V, L236F

Promoter

pTlpA and pTlpA, LacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP16

Plasmid

#22595

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP16 (USP16 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet

Plasmid

#138086

Purpose

Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles

Depositor

Eva Frickel

Article

Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with heptamerized tet‐operat…

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApaLID211A-HA

Plasmid

#211459

Purpose

Express mitochondrial localized ApaLI with D211A mutation

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-D211A

Use

Tags

HA

Expression

Mammalian

Mutation

D211A

Promoter

CMV

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-dTAG

Plasmid

#207694

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

Depositor

Laurence Pelletier

Article

Philip et al bioRxiv

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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