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pcDNA5-FRT-TO-FH-Nsp7
Plasmid#157689Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp7 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-Nsp15-HF
Plasmid#157722Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoterDepositorInsertNsp15-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSCV N-term HA-FLAG puro BRD7 shRescue
Plasmid#41936DepositorInsertBRD7 (BRD7 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-hMeCP2-R111G
Plasmid#48085PurposeMammalian expression of Gal4 tagged MeCP2-WT-R111GDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGal4ExpressionMammalianMutationR111G mutant MeCP2PromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + S608
Plasmid#58913Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with S608 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABE (hygro) hYAP 1-1delta
Plasmid#124150PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCB320-sgNC.SPA
Plasmid#169834PurposeExpresses a negative control sgRNADepositorInsertsafe harbor sgRNA
UseCRISPR, Lentiviral, and Mouse TargetingTagsExpressionMammalianMutationPromotermU6Available sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCB320-sgNC.ACOCA
Plasmid#169836PurposeExpresses a negative control sgRNADepositorInsertsafe harbor sgRNA
UseCRISPR, Lentiviral, and Mouse TargetingTagsExpressionMammalianMutationPromotermU6Available sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRb delta CDK + S230
Plasmid#58907Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with S230 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + T252
Plasmid#58910Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with T252 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Gal4-entry
Plasmid#128014PurposeVector containing the Gal4-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertGal4-DBD-3xFlag
UseGal4 entry vectorTagsGal4-DBD-3xFlagExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SNORD27h_115mut
Plasmid#73068Purposeexpression clone for human mutated SNORD27 (C/D box snoRNA U27)DepositorInsertSNORD27 (SNORD27 Human)
UseTagsno tagExpressionMammalianMutationexpression cassette for SNORD27 with AS box from …PromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-hMeCP2-G273X
Plasmid#48084PurposeMammalian expression of Gal4 tagged MeCP2-G273XDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGal4ExpressionMammalianMutationexpresses AT-Hook G273XPromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp2
Plasmid#157682Purposemammalian expression of untagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoterDepositorInsertNsp2 (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-Nsp2-HF
Plasmid#157684Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoterDepositorInsertNsp2-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1 (-) hYAP 1-2beta
Plasmid#124144PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 (-) hYAP 1-1gamma
Plasmid#124141PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
ABE8.13-d
Plasmid#136297Purposeexpresses ABE8.13-d in mammalian cellsDepositorInsertABE8.13-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only
