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Plasmid#205055PurposeTWNK protein expressionDepositorInsertTWNK (TWNK Human)
UseTagsExpressionBacterialMutationLacks N terminal amino acid from 1-359PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EYFP Cav2.2 pRK5
Plasmid#206099Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal EYFP tag and an exofacial double HA tag in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pASPIre4
Plasmid#196656PurposeDerivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.DepositorInsertBxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)
UseTagsExpressionBacterialMutationSpeI site in Bxb1 CDSPromoterrhamnose-inducible promoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_tKiMBImut-T2A-caMEK
Plasmid#199579PurposeExpress tKiMBImut(AA) and caMEK in an AAV vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator (mutant)
constitutively active MEK
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-dCas13b-ADAR2DD
Plasmid#190913PurposeAddgene #103871 with 3x FLAG on N-terminal of dCas13DepositorInsert3X FLAG
UseCRISPRTags3X FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG.Myc-SARS2_3CL(C145A)
Plasmid#200121PurposeExpresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cellsDepositorInsertSARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)
UseTagsMycExpressionMammalianMutationC145APromoterCAGAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM4-Flag
Plasmid#180409Purposelentiviral transduction of human TGM4 (with a C-terminal Flag tag) geneDepositorInsertTGM4 (TGM4 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM5-V5
Plasmid#180414Purposelentiviral transduction of human TGM5 (with a C-terminal V5 tag) geneDepositorInsertTGM5 (TGM5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-HistoneH2B-L2
Plasmid#186357PurposeEntry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertHistone H2B (H2BC11 Human)
UseExpression of a tagged nuclear markerTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET303-ColE1-TR-E192C-del1-40
Plasmid#180235PurposeExpresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364) with C terminal cysteine for maleimide chemistryDepositorInsertColicin E1
UseTags6x His-tagExpressionBacterialMutationTruncation of ColE1 containing T and R domains (r…PromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_P41-Cd4-bio-His
Plasmid#126847PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertP41 (PKH_030970 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_P92-Cd4-bio-His
Plasmid#126848PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertP92 (PKH_110660 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_MSP10-Cd4-bio-His
Plasmid#126940PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertMSP10 (PKH_112880 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_GAMA-Cd4-bio-His
Plasmid#126842PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertGAMA (PKH_050210 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_MSP4-Cd4-bio-His
Plasmid#126843PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertMSP4 (PKH_041300 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_MSP5-Cd4-bio-His
Plasmid#126844PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertMSP5 (PKH_041310 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_P12-Cd4-bio-His
Plasmid#126845PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertP12 (PKH_113620 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_P38-Cd4-bio-His
Plasmid#126846PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertP38 (PKH_102490 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pm_P12-Cd4-bio-His
Plasmid#126822PurposeExpression of recombinant P. malariae protein in mammalian cellsDepositorInsertP12 (P12p Plasmodium malariae)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis
Plasmid#175801PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184Q W330YPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only