Addgene

We narrowed to 18,174 results for: OMP;

Showing: 7341 - 7360 of 18174 results

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pET21-pelB-LaM-6

Plasmid

#172771

Purpose

Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-6

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGB Tnos:NptII:Pnos (GB0226)

Plasmid

#68212

Purpose

Transcriptional unit for kanamycin resistance gene plant expression

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

NptII

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-12-C, 21-G

Plasmid

#124683

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (1-170)

Plasmid

#203426

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Use

Tags

2 x HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (1-208)

Plasmid

#203427

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Use

Tags

2 x HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (1-277)

Plasmid

#203428

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Use

Tags

2 x HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (170-439)

Plasmid

#203430

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Use

Tags

2 x HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB4

Plasmid

#19526

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DnaJ (Hsp40) homolog, subfamily B, member 4 (DNAJB4 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pKMD106h - intein-Cas9(C574)

Plasmid

#64191

Purpose

Expresses intein-Cas9(C574) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMGIB-3xFLAG-reptin

Plasmid

#53609

Purpose

Retroviral expression of human reptin

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

reptin (RUVBL2 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

CDS mutated to confer resistance to shRNA. All mu…

Promoter

LTR

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pHH0103_VAV2-2/2

Plasmid

#91506

Purpose

Protein expression and purification of human SH3 domain construct VAV2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

VAV2-2/2 (VAV2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

Plasmid

#84258

Purpose

Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

Sp sgTRE3G
PYL1-VPR

Use

CRISPR and Lentiviral

Tags

IRES-mCherry and PYL1

Expression

Mammalian

Mutation

Promoter

CMV and mouse U6

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPH3

Plasmid

#19482

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPH3 (HSPA4L Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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LentiU6-hNEUROD1 gRNA_1-MS2-Puro

Plasmid

#192676

Purpose

Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human NEUROD1 activating gRNA #1 (NEUROD1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-eGFP

Plasmid

#157734

Purpose

mammalian expression of N-terminally Flag-His8 tagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-eGFP

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Flag WDR24 pLJM1

Plasmid

#46337

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR24 (WDR24 Human)

Use

Lentiviral

Tags

FLag

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-G12-CheRiff-T2A-mCherry

Plasmid

#170275

Purpose

CheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

CheRiff-T2A-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

11xUAS with TATA-box minimal promoter

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eYFP-f

Plasmid

#104057

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequence

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

EYFP-f

Use

AAV

Tags

Expression

Mutation

Promoter

TRE

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Gap43

Plasmid

#74336

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.

Depositor

Peter Mombaerts

Article

Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

Insert

growth associated protein 43 (Gap43 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pJZC42

Plasmid

#66564

Purpose

sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

Depositor

Stanley Qi, Wendell Lim

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1XPP7
PCP-VP64 IRES mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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